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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nostoc punctiforme Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP450535NHQ
CSB-EP450535NHQ
CSB-BP450535NHQ
CSB-MP450535NHQ
CSB-EP450535NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP450536NHQ
CSB-EP450536NHQ
CSB-BP450536NHQ
CSB-MP450536NHQ
CSB-EP450536NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme UPF0758 protein Npun_R6401 (Npun_R6401) CSB-YP450537NHQ
CSB-EP450537NHQ
CSB-BP450537NHQ
CSB-MP450537NHQ
CSB-EP450537NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glutamate--tRNA ligase (gltX) CSB-YP450538NHQ
CSB-EP450538NHQ
CSB-BP450538NHQ
CSB-MP450538NHQ
CSB-EP450538NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP450540NHQ
CSB-EP450540NHQ
CSB-BP450540NHQ
CSB-MP450540NHQ
CSB-EP450540NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 30S ribosomal protein S18 (rpsR) CSB-YP450541NHQ
CSB-EP450541NHQ
CSB-BP450541NHQ
CSB-MP450541NHQ
CSB-EP450541NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Elongation factor 4 (lepA), partial CSB-YP450542NHQ
CSB-EP450542NHQ
CSB-BP450542NHQ
CSB-MP450542NHQ
CSB-EP450542NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP450543NHQ
CSB-EP450543NHQ
CSB-BP450543NHQ
CSB-MP450543NHQ
CSB-EP450543NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP450544NHQ
CSB-EP450544NHQ
CSB-BP450544NHQ
CSB-MP450544NHQ
CSB-EP450544NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Chorismate synthase (aroC) CSB-YP450545NHQ
CSB-EP450545NHQ
CSB-BP450545NHQ
CSB-MP450545NHQ
CSB-EP450545NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Chromosomal replication initiator protein DnaA (dnaA) CSB-YP450546NHQ
CSB-EP450546NHQ
CSB-BP450546NHQ
CSB-MP450546NHQ
CSB-EP450546NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Cell division topological specificity factor (minE) CSB-YP450547NHQ
CSB-EP450547NHQ
CSB-BP450547NHQ
CSB-MP450547NHQ
CSB-EP450547NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Cytochrome b6-f complex iron-sulfur subunit (petC), partial CSB-YP450548NHQ1
CSB-EP450548NHQ1
CSB-BP450548NHQ1
CSB-MP450548NHQ1
CSB-EP450548NHQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glycogen synthase (glgA) CSB-YP450549NHQ
CSB-EP450549NHQ
CSB-BP450549NHQ
CSB-MP450549NHQ
CSB-EP450549NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme UPF0296 protein Npun_R3866 (Npun_R3866) CSB-YP450550NHQ
CSB-EP450550NHQ
CSB-BP450550NHQ
CSB-MP450550NHQ
CSB-EP450550NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP450551NHQ
CSB-EP450551NHQ
CSB-BP450551NHQ
CSB-MP450551NHQ
CSB-EP450551NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP450552NHQ
CSB-EP450552NHQ
CSB-BP450552NHQ
CSB-MP450552NHQ
CSB-EP450552NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme UvrABC system protein C (uvrC), partial CSB-YP450553NHQ
CSB-EP450553NHQ
CSB-BP450553NHQ
CSB-MP450553NHQ
CSB-EP450553NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP450554NHQ
CSB-EP450554NHQ
CSB-BP450554NHQ
CSB-MP450554NHQ
CSB-EP450554NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme NAD (P)H-quinone oxidoreductase subunit 3, partial CSB-YP450555NHQ1
CSB-EP450555NHQ1
CSB-BP450555NHQ1
CSB-MP450555NHQ1
CSB-EP450555NHQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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