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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bartonella tribocorum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP435364BOS
CSB-EP435364BOS
CSB-BP435364BOS
CSB-MP435364BOS
CSB-EP435364BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Ribosome-binding factor A (rbfA) CSB-YP435365BOS
CSB-EP435365BOS
CSB-BP435365BOS
CSB-MP435365BOS
CSB-EP435365BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP435366BOS
CSB-EP435366BOS
CSB-BP435366BOS
CSB-MP435366BOS
CSB-EP435366BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP435367BPB
CSB-EP435367BPB
CSB-BP435367BPB
CSB-MP435367BPB
CSB-EP435367BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii D-amino acid dehydrogenase small subunit (dadA) CSB-YP435368BPB
CSB-EP435368BPB
CSB-BP435368BPB
CSB-MP435368BPB
CSB-EP435368BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 30S ribosomal protein S20 (rpsT) CSB-YP435369BPB
CSB-EP435369BPB
CSB-BP435369BPB
CSB-MP435369BPB
CSB-EP435369BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum GTPase Der (der) CSB-YP435370BOS
CSB-EP435370BOS
CSB-BP435370BOS
CSB-MP435370BOS
CSB-EP435370BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Chorismate synthase (aroC) CSB-YP435371BOS
CSB-EP435371BOS
CSB-BP435371BOS
CSB-MP435371BOS
CSB-EP435371BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Lysine--tRNA ligase (lysS), partial CSB-YP435372BPB
CSB-EP435372BPB
CSB-BP435372BPB
CSB-MP435372BPB
CSB-EP435372BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Trigger factor (tig) CSB-YP435373BPB
CSB-EP435373BPB
CSB-BP435373BPB
CSB-MP435373BPB
CSB-EP435373BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Thiazole synthase (thiG) CSB-YP435374BOS
CSB-EP435374BOS
CSB-BP435374BOS
CSB-MP435374BOS
CSB-EP435374BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Orotate phosphoribosyltransferase (pyrE) CSB-YP435375BOS
CSB-EP435375BOS
CSB-BP435375BOS
CSB-MP435375BOS
CSB-EP435375BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 30S ribosomal protein S18 (rpsR) CSB-YP435376BOS
CSB-EP435376BOS
CSB-BP435376BOS
CSB-MP435376BOS
CSB-EP435376BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP435377BOS
CSB-EP435377BOS
CSB-BP435377BOS
CSB-MP435377BOS
CSB-EP435377BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Trigger factor (tig) CSB-YP435378BOS
CSB-EP435378BOS
CSB-BP435378BOS
CSB-MP435378BOS
CSB-EP435378BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 30S ribosomal protein S2 (rpsB) CSB-YP435379BOS
CSB-EP435379BOS
CSB-BP435379BOS
CSB-MP435379BOS
CSB-EP435379BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Bifunctional protein FolD (folD) CSB-YP435380BPB
CSB-EP435380BPB
CSB-BP435380BPB
CSB-MP435380BPB
CSB-EP435380BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii GTP cyclohydrolase folE2 (folE2) CSB-YP435381BPB
CSB-EP435381BPB
CSB-BP435381BPB
CSB-MP435381BPB
CSB-EP435381BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP435382BPB
CSB-EP435382BPB
CSB-BP435382BPB
CSB-MP435382BPB
CSB-EP435382BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UPF0758 protein Bpet3149 (Bpet3149) CSB-YP435383BPB
CSB-EP435383BPB
CSB-BP435383BPB
CSB-MP435383BPB
CSB-EP435383BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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