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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S11 (rpsK) CSB-YP450295FLX
CSB-EP450295FLX
CSB-BP450295FLX
CSB-MP450295FLX
CSB-EP450295FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Trigger factor (tig) CSB-YP450296FLX
CSB-EP450296FLX
CSB-BP450296FLX
CSB-MP450296FLX
CSB-EP450296FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UPF0133 protein Smlt1015 (Smlt1015) CSB-YP450297FLX
CSB-EP450297FLX
CSB-BP450297FLX
CSB-MP450297FLX
CSB-EP450297FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP450298FLX
CSB-EP450298FLX
CSB-BP450298FLX
CSB-MP450298FLX
CSB-EP450298FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Aspartate carbamoyltransferase (pyrB) CSB-YP450299FLX
CSB-EP450299FLX
CSB-BP450299FLX
CSB-MP450299FLX
CSB-EP450299FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia tRNA (guanine-N (7)-)-methyltransferase CSB-YP450300FLX
CSB-EP450300FLX
CSB-BP450300FLX
CSB-MP450300FLX
CSB-EP450300FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Ribonuclease PH (rph) CSB-YP450301FLX
CSB-EP450301FLX
CSB-BP450301FLX
CSB-MP450301FLX
CSB-EP450301FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia DNA replication and repair protein recF (recF) CSB-YP450302FLX
CSB-EP450302FLX
CSB-BP450302FLX
CSB-MP450302FLX
CSB-EP450302FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia GTPase obg (obg) CSB-YP450303FLX
CSB-EP450303FLX
CSB-BP450303FLX
CSB-MP450303FLX
CSB-EP450303FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glucans biosynthesis protein D (opgD), partial CSB-YP450304FLX
CSB-EP450304FLX
CSB-BP450304FLX
CSB-MP450304FLX
CSB-EP450304FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia ATP-dependent protease subunit HslV (hslV) CSB-YP450305FLX
CSB-EP450305FLX
CSB-BP450305FLX
CSB-MP450305FLX
CSB-EP450305FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L9 (rplI) CSB-YP450306LMV
CSB-EP450306LMV
CSB-BP450306LMV
CSB-MP450306LMV
CSB-EP450306LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP450307LMV
CSB-EP450307LMV
CSB-BP450307LMV
CSB-MP450307LMV
CSB-EP450307LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Bifunctional protein GlmU (glmU) CSB-YP450308LMV
CSB-EP450308LMV
CSB-BP450308LMV
CSB-MP450308LMV
CSB-EP450308LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Peptidyl-tRNA hydrolase (pth) CSB-YP450309LMV
CSB-EP450309LMV
CSB-BP450309LMV
CSB-MP450309LMV
CSB-EP450309LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Large-conductance mechanosensitive channel (mscL), partial CSB-YP450310LMV1
CSB-EP450310LMV1
CSB-BP450310LMV1
CSB-MP450310LMV1
CSB-EP450310LMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Protein translocase subunit SecA (secA), partial CSB-YP450311LMV
CSB-EP450311LMV
CSB-BP450311LMV
CSB-MP450311LMV
CSB-EP450311LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri SsrA-binding protein (smpB) CSB-YP450312LMV
CSB-EP450312LMV
CSB-BP450312LMV
CSB-MP450312LMV
CSB-EP450312LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Arginine deiminase (arcA) CSB-YP450313LMV
CSB-EP450313LMV
CSB-BP450313LMV
CSB-MP450313LMV
CSB-EP450313LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Putative competence-damage inducible protein (cinA) CSB-YP450314LMV
CSB-EP450314LMV
CSB-BP450314LMV
CSB-MP450314LMV
CSB-EP450314LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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