Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum UPF0758 protein CLH_0547 (CLH_0547) CSB-YP451096DUF
CSB-EP451096DUF
CSB-BP451096DUF
CSB-MP451096DUF
CSB-EP451096DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP451097DUF
CSB-EP451097DUF
CSB-BP451097DUF
CSB-MP451097DUF
CSB-EP451097DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative phosphotransferase CLH_3298 (CLH_3298) CSB-YP451098DUF
CSB-EP451098DUF
CSB-BP451098DUF
CSB-MP451098DUF
CSB-EP451098DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA mismatch repair protein MutS (mutS), partial CSB-YP451099DUF
CSB-EP451099DUF
CSB-BP451099DUF
CSB-MP451099DUF
CSB-EP451099DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP451100DUF
CSB-EP451100DUF
CSB-BP451100DUF
CSB-MP451100DUF
CSB-EP451100DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Heat-inducible transcription repressor HrcA (hrcA) CSB-YP451101DUF
CSB-EP451101DUF
CSB-BP451101DUF
CSB-MP451101DUF
CSB-EP451101DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Xanthine phosphoribosyltransferase (xpt) CSB-YP451102DUF
CSB-EP451102DUF
CSB-BP451102DUF
CSB-MP451102DUF
CSB-EP451102DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP451103DUF
CSB-EP451103DUF
CSB-BP451103DUF
CSB-MP451103DUF
CSB-EP451103DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphopantetheine adenylyltransferase (coaD) CSB-YP451104DUF
CSB-EP451104DUF
CSB-BP451104DUF
CSB-MP451104DUF
CSB-EP451104DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA (guanine-N (1)-)-methyltransferase CSB-YP451105DUF
CSB-EP451105DUF
CSB-BP451105DUF
CSB-MP451105DUF
CSB-EP451105DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosome-binding factor A (rbfA) CSB-YP451106DUF
CSB-EP451106DUF
CSB-BP451106DUF
CSB-MP451106DUF
CSB-EP451106DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 5-dehydro-2-deoxygluconokinase (iolC) CSB-YP451107DUF
CSB-EP451107DUF
CSB-BP451107DUF
CSB-MP451107DUF
CSB-EP451107DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP451108DUF
CSB-EP451108DUF
CSB-BP451108DUF
CSB-MP451108DUF
CSB-EP451108DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 33 kDa chaperonin (hslO) CSB-YP451109DUF
CSB-EP451109DUF
CSB-BP451109DUF
CSB-MP451109DUF
CSB-EP451109DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. UPF0133 protein SYO3AOP1_1366 (SYO3AOP1_1366) CSB-YP451110FPP
CSB-EP451110FPP
CSB-BP451110FPP
CSB-MP451110FPP
CSB-EP451110FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Pantothenate synthetase (panC) CSB-YP451111FPP
CSB-EP451111FPP
CSB-BP451111FPP
CSB-MP451111FPP
CSB-EP451111FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP451112FPP
CSB-EP451112FPP
CSB-BP451112FPP
CSB-MP451112FPP
CSB-EP451112FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. CinA-like protein (SYO3AOP1_0125) CSB-YP451113FPP
CSB-EP451113FPP
CSB-BP451113FPP
CSB-MP451113FPP
CSB-EP451113FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Glutamyl-tRNA reductase (hemA) CSB-YP451114FPP
CSB-EP451114FPP
CSB-BP451114FPP
CSB-MP451114FPP
CSB-EP451114FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP451115FPP
CSB-EP451115FPP
CSB-BP451115FPP
CSB-MP451115FPP
CSB-EP451115FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>