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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter pylori Pantothenate synthetase (panC) CSB-YP451076HUZ
CSB-EP451076HUZ
CSB-BP451076HUZ
CSB-MP451076HUZ
CSB-EP451076HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease subunit alpha (ureA) CSB-YP451077HUZ
CSB-EP451077HUZ
CSB-BP451077HUZ
CSB-MP451077HUZ
CSB-EP451077HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartate carbamoyltransferase (pyrB) CSB-YP451078DUF
CSB-EP451078DUF
CSB-BP451078DUF
CSB-MP451078DUF
CSB-EP451078DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum V-type ATP synthase alpha chain (atpA), partial CSB-YP451079DUF
CSB-EP451079DUF
CSB-BP451079DUF
CSB-MP451079DUF
CSB-EP451079DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP451080DUF
CSB-EP451080DUF
CSB-BP451080DUF
CSB-MP451080DUF
CSB-EP451080DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA replication and repair protein recF (recF) CSB-YP451081DUF
CSB-EP451081DUF
CSB-BP451081DUF
CSB-MP451081DUF
CSB-EP451081DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP451082DUF
CSB-EP451082DUF
CSB-BP451082DUF
CSB-MP451082DUF
CSB-EP451082DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Asparagine--tRNA ligase (asnS) CSB-YP451083DUF
CSB-EP451083DUF
CSB-BP451083DUF
CSB-MP451083DUF
CSB-EP451083DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP451084DUF
CSB-EP451084DUF
CSB-BP451084DUF
CSB-MP451084DUF
CSB-EP451084DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Hydroxyethylthiazole kinase (thiM) CSB-YP451085DUF
CSB-EP451085DUF
CSB-BP451085DUF
CSB-MP451085DUF
CSB-EP451085DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L1 (rplA) CSB-YP451086DUF
CSB-EP451086DUF
CSB-BP451086DUF
CSB-MP451086DUF
CSB-EP451086DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L4 (rplD) CSB-YP451087DUF
CSB-EP451087DUF
CSB-BP451087DUF
CSB-MP451087DUF
CSB-EP451087DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S17 (rpsQ) CSB-YP451088DUF
CSB-EP451088DUF
CSB-BP451088DUF
CSB-MP451088DUF
CSB-EP451088DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L15 (rplO) CSB-YP451089DUF
CSB-EP451089DUF
CSB-BP451089DUF
CSB-MP451089DUF
CSB-EP451089DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L17 (rplQ) CSB-YP451090DUF
CSB-EP451090DUF
CSB-BP451090DUF
CSB-MP451090DUF
CSB-EP451090DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP451091DUF
CSB-EP451091DUF
CSB-BP451091DUF
CSB-MP451091DUF
CSB-EP451091DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP451092DUF
CSB-EP451092DUF
CSB-BP451092DUF
CSB-MP451092DUF
CSB-EP451092DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP451093DUF
CSB-EP451093DUF
CSB-BP451093DUF
CSB-MP451093DUF
CSB-EP451093DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum D-ribose pyranase (rbsD) CSB-YP451094DUF
CSB-EP451094DUF
CSB-BP451094DUF
CSB-MP451094DUF
CSB-EP451094DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UvrABC system protein B (uvrB), partial CSB-YP451095DUF
CSB-EP451095DUF
CSB-BP451095DUF
CSB-MP451095DUF
CSB-EP451095DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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