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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chloroflexus aurantiacus Protein translocase subunit SecA (secA), partial CSB-YP435964DSX
CSB-EP435964DSX
CSB-BP435964DSX
CSB-MP435964DSX
CSB-EP435964DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Ribosome maturation factor RimM (rimM) CSB-YP435965DSX
CSB-EP435965DSX
CSB-BP435965DSX
CSB-MP435965DSX
CSB-EP435965DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP435966DSX
CSB-EP435966DSX
CSB-BP435966DSX
CSB-MP435966DSX
CSB-EP435966DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Cell division protein FtsQ (ftsQ), partial CSB-YP435967DSX
CSB-EP435967DSX
CSB-BP435967DSX
CSB-MP435967DSX
CSB-EP435967DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP435968DSX
CSB-EP435968DSX
CSB-BP435968DSX
CSB-MP435968DSX
CSB-EP435968DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus ATP synthase epsilon chain (atpC) CSB-YP435969DSX
CSB-EP435969DSX
CSB-BP435969DSX
CSB-MP435969DSX
CSB-EP435969DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP435970DSX
CSB-EP435970DSX
CSB-BP435970DSX
CSB-MP435970DSX
CSB-EP435970DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 50S ribosomal protein L16 (rplP) CSB-YP435971DSX
CSB-EP435971DSX
CSB-BP435971DSX
CSB-MP435971DSX
CSB-EP435971DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 50S ribosomal protein L15 (rplO) CSB-YP435972DSX
CSB-EP435972DSX
CSB-BP435972DSX
CSB-MP435972DSX
CSB-EP435972DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 50S ribosomal protein L13 (rplM) CSB-YP435973DSX
CSB-EP435973DSX
CSB-BP435973DSX
CSB-MP435973DSX
CSB-EP435973DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus GTPase Der (der) CSB-YP435974DSX
CSB-EP435974DSX
CSB-BP435974DSX
CSB-MP435974DSX
CSB-EP435974DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP435975DSX
CSB-EP435975DSX
CSB-BP435975DSX
CSB-MP435975DSX
CSB-EP435975DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP435976DSX
CSB-EP435976DSX
CSB-BP435976DSX
CSB-MP435976DSX
CSB-EP435976DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 60 kDa chaperonin 1 (groL1), partial CSB-YP435977DSX
CSB-EP435977DSX
CSB-BP435977DSX
CSB-MP435977DSX
CSB-EP435977DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Octanoyltransferase (lipB) CSB-YP435978DSX
CSB-EP435978DSX
CSB-BP435978DSX
CSB-MP435978DSX
CSB-EP435978DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Large-conductance mechanosensitive channel (mscL), partial CSB-YP435979RIH1
CSB-EP435979RIH1
CSB-BP435979RIH1
CSB-MP435979RIH1
CSB-EP435979RIH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP435980RIH
CSB-EP435980RIH
CSB-BP435980RIH
CSB-MP435980RIH
CSB-EP435980RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP435981RIH
CSB-EP435981RIH
CSB-BP435981RIH
CSB-MP435981RIH
CSB-EP435981RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Ribosome-binding factor A (rbfA) CSB-YP435982RIH
CSB-EP435982RIH
CSB-BP435982RIH
CSB-MP435982RIH
CSB-EP435982RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP435983RIH
CSB-EP435983RIH
CSB-BP435983RIH
CSB-MP435983RIH
CSB-EP435983RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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