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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Podospora anserina Nuclear distribution protein PAC1-1 (PAC1-1) CSB-YP450255EXJ
CSB-EP450255EXJ
CSB-BP450255EXJ
CSB-MP450255EXJ
CSB-EP450255EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP450256DZS
CSB-EP450256DZS
CSB-BP450256DZS
CSB-MP450256DZS
CSB-EP450256DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis UPF0391 membrane protein RALTA_A0099 (RALTA_A0099), partial CSB-YP450257DZS
CSB-EP450257DZS
CSB-BP450257DZS
CSB-MP450257DZS
CSB-EP450257DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Diaminopimelate epimerase (dapF) CSB-YP450258DZS
CSB-EP450258DZS
CSB-BP450258DZS
CSB-MP450258DZS
CSB-EP450258DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Bifunctional protein GlmU (glmU) CSB-YP450259DZS
CSB-EP450259DZS
CSB-BP450259DZS
CSB-MP450259DZS
CSB-EP450259DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 50S ribosomal protein L13 (rplM) CSB-YP450260DZS
CSB-EP450260DZS
CSB-BP450260DZS
CSB-MP450260DZS
CSB-EP450260DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP450261DZS
CSB-EP450261DZS
CSB-BP450261DZS
CSB-MP450261DZS
CSB-EP450261DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP450262DZS
CSB-EP450262DZS
CSB-BP450262DZS
CSB-MP450262DZS
CSB-EP450262DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Mannitol-1-phosphate 5-dehydrogenase (Pa_6_10260) CSB-YP450263EXJ
CSB-EP450263EXJ
CSB-BP450263EXJ
CSB-MP450263EXJ
CSB-EP450263EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Bifunctional lycopene cyclase/phytoene synthase, partial CSB-YP450264EXJ1
CSB-EP450264EXJ1
CSB-BP450264EXJ1
CSB-MP450264EXJ1
CSB-EP450264EXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina 3-ketoacyl-CoA reductase (Pa_6_6580), partial CSB-YP450265EXJ1
CSB-EP450265EXJ1
CSB-BP450265EXJ1
CSB-MP450265EXJ1
CSB-EP450265EXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina 40S ribosomal protein S1 (RPS1) CSB-YP450266EXJ
CSB-EP450266EXJ
CSB-BP450266EXJ
CSB-MP450266EXJ
CSB-EP450266EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Nuclear distribution protein PAC1-2 (PAC1-2) CSB-YP450267EXJ
CSB-EP450267EXJ
CSB-BP450267EXJ
CSB-MP450267EXJ
CSB-EP450267EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP450268ROM
CSB-EP450268ROM
CSB-BP450268ROM
CSB-MP450268ROM
CSB-EP450268ROM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquareovirus G Non-structural protein 1 (S4), partial CSB-YP450269DNU
CSB-EP450269DNU
CSB-BP450269DNU
CSB-MP450269DNU
CSB-EP450269DNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Austrelaps labialis Protease inhibitor CSB-YP450270DPM
CSB-EP450270DPM
CSB-BP450270DPM
CSB-MP450270DPM
CSB-EP450270DPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus caracteristicus Conotoxin Ca15a CSB-YP450271DVW
CSB-EP450271DVW
CSB-BP450271DVW
CSB-MP450271DVW
CSB-EP450271DVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phomopsis amygdali Copalyl diphosphate synthase (PaDC2), partial CSB-YP450272EUU
CSB-EP450272EUU
CSB-BP450272EUU
CSB-MP450272EUU
CSB-EP450272EUU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine/threonine-protein kinase BRSK1 (Brsk1) CSB-YP450273RA
CSB-EP450273RA
CSB-BP450273RA
CSB-MP450273RA
CSB-EP450273RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UPF0234 protein Smlt4090 (Smlt4090) CSB-YP450274FLX
CSB-EP450274FLX
CSB-BP450274FLX
CSB-MP450274FLX
CSB-EP450274FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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