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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia ambifaria 50S ribosomal protein L34 (rpmH) CSB-YP450155BXP
CSB-EP450155BXP
CSB-BP450155BXP
CSB-MP450155BXP
CSB-EP450155BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria ATP synthase epsilon chain (atpC) CSB-YP450156BXP
CSB-EP450156BXP
CSB-BP450156BXP
CSB-MP450156BXP
CSB-EP450156BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Histidine--tRNA ligase (hisS) CSB-YP450157BXP
CSB-EP450157BXP
CSB-BP450157BXP
CSB-MP450157BXP
CSB-EP450157BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP450158BXP
CSB-EP450158BXP
CSB-BP450158BXP
CSB-MP450158BXP
CSB-EP450158BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L2 (rplB) CSB-YP450159BXP
CSB-EP450159BXP
CSB-BP450159BXP
CSB-MP450159BXP
CSB-EP450159BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Recombination protein RecR (recR) CSB-YP450160BXP
CSB-EP450160BXP
CSB-BP450160BXP
CSB-MP450160BXP
CSB-EP450160BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP450161BXP
CSB-EP450161BXP
CSB-BP450161BXP
CSB-MP450161BXP
CSB-EP450161BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L6 (rplF) CSB-YP450162BXP
CSB-EP450162BXP
CSB-BP450162BXP
CSB-MP450162BXP
CSB-EP450162BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S11 (rpsK) CSB-YP450163BXP
CSB-EP450163BXP
CSB-BP450163BXP
CSB-MP450163BXP
CSB-EP450163BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria ATP phosphoribosyltransferase (hisG) CSB-YP450164BXP
CSB-EP450164BXP
CSB-BP450164BXP
CSB-MP450164BXP
CSB-EP450164BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Lipid-A-disaccharide synthase (lpxB) CSB-YP450165BXP
CSB-EP450165BXP
CSB-BP450165BXP
CSB-MP450165BXP
CSB-EP450165BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria [Protein-PII] uridylyltransferase (glnD), partial CSB-YP450166BXP
CSB-EP450166BXP
CSB-BP450166BXP
CSB-MP450166BXP
CSB-EP450166BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Glutamate--tRNA ligase (gltX) CSB-YP450167BXP
CSB-EP450167BXP
CSB-BP450167BXP
CSB-MP450167BXP
CSB-EP450167BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP450168BXP
CSB-EP450168BXP
CSB-BP450168BXP
CSB-MP450168BXP
CSB-EP450168BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L27 (rpmA) CSB-YP450169BXP
CSB-EP450169BXP
CSB-BP450169BXP
CSB-MP450169BXP
CSB-EP450169BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Enolase (eno) CSB-YP450170BXP
CSB-EP450170BXP
CSB-BP450170BXP
CSB-MP450170BXP
CSB-EP450170BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Leucine--tRNA ligase (leuS), partial CSB-YP450171BXP
CSB-EP450171BXP
CSB-BP450171BXP
CSB-MP450171BXP
CSB-EP450171BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP450172BXP
CSB-EP450172BXP
CSB-BP450172BXP
CSB-MP450172BXP
CSB-EP450172BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Chaperone protein DnaJ (dnaJ) CSB-YP450173BXP
CSB-EP450173BXP
CSB-BP450173BXP
CSB-MP450173BXP
CSB-EP450173BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S15 (rpsO) CSB-YP450174BXP
CSB-EP450174BXP
CSB-BP450174BXP
CSB-MP450174BXP
CSB-EP450174BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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