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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB NADH pyrophosphatase (nudC) CSB-YP450616YAW
CSB-EP450616YAW
CSB-BP450616YAW
CSB-MP450616YAW
CSB-EP450616YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP450617YAW
CSB-EP450617YAW
CSB-BP450617YAW
CSB-MP450617YAW
CSB-EP450617YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Divalent-cation tolerance protein CutA (cutA) CSB-YP450618YAW
CSB-EP450618YAW
CSB-BP450618YAW
CSB-MP450618YAW
CSB-EP450618YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB DNA mismatch repair protein mutL (mutL), partial CSB-YP450619YAW
CSB-EP450619YAW
CSB-BP450619YAW
CSB-MP450619YAW
CSB-EP450619YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP450620YAW
CSB-EP450620YAW
CSB-BP450620YAW
CSB-MP450620YAW
CSB-EP450620YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 30S ribosomal protein S21 (rpsU) CSB-YP450621YAW
CSB-EP450621YAW
CSB-BP450621YAW
CSB-MP450621YAW
CSB-EP450621YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Arginine repressor (argR) CSB-YP450622YAW
CSB-EP450622YAW
CSB-BP450622YAW
CSB-MP450622YAW
CSB-EP450622YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP450623YAW
CSB-EP450623YAW
CSB-BP450623YAW
CSB-MP450623YAW
CSB-EP450623YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Autoinducer 2 import system permease protein lsrC (lsrC), partial CSB-YP450624YAW
CSB-EP450624YAW
CSB-BP450624YAW
CSB-MP450624YAW
CSB-EP450624YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphopentomutase (deoB) CSB-YP450625YAW
CSB-EP450625YAW
CSB-BP450625YAW
CSB-MP450625YAW
CSB-EP450625YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0246 protein YPTS_0629 (YPTS_0629) CSB-YP450626YAW
CSB-EP450626YAW
CSB-BP450626YAW
CSB-MP450626YAW
CSB-EP450626YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Isoleucine--tRNA ligase (ileS), partial CSB-YP450627YAW
CSB-EP450627YAW
CSB-BP450627YAW
CSB-MP450627YAW
CSB-EP450627YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Tryptophan synthase alpha chain (trpA) CSB-YP450628YAW
CSB-EP450628YAW
CSB-BP450628YAW
CSB-MP450628YAW
CSB-EP450628YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0042 nucleotide-binding protein YPTS_3715 (YPTS_3715) CSB-YP450629YAW
CSB-EP450629YAW
CSB-BP450629YAW
CSB-MP450629YAW
CSB-EP450629YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB DNA-binding protein fis (fis) CSB-YP450630YAW
CSB-EP450630YAW
CSB-BP450630YAW
CSB-MP450630YAW
CSB-EP450630YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP450631YAW
CSB-EP450631YAW
CSB-BP450631YAW
CSB-MP450631YAW
CSB-EP450631YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0231 protein YPTS_0747 (YPTS_0747) CSB-YP450632YAW
CSB-EP450632YAW
CSB-BP450632YAW
CSB-MP450632YAW
CSB-EP450632YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Electron transport complex protein RnfC (rnfC), partial CSB-YP450633YAW
CSB-EP450633YAW
CSB-BP450633YAW
CSB-MP450633YAW
CSB-EP450633YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein psiE homolog (psiE), partial CSB-YP450634YAW1
CSB-EP450634YAW1
CSB-BP450634YAW1
CSB-MP450634YAW1
CSB-EP450634YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L36 2 (rpmJ2) CSB-YP450635YAW
CSB-EP450635YAW
CSB-BP450635YAW
CSB-MP450635YAW
CSB-EP450635YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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