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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leptothrix cholodnii 50S ribosomal protein L34 (rpmH) CSB-YP452057LOT
CSB-EP452057LOT
CSB-BP452057LOT
CSB-MP452057LOT
CSB-EP452057LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii UPF0758 protein Lcho_0695 (Lcho_0695) CSB-YP452058LOT
CSB-EP452058LOT
CSB-BP452058LOT
CSB-MP452058LOT
CSB-EP452058LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP452059LOT
CSB-EP452059LOT
CSB-BP452059LOT
CSB-MP452059LOT
CSB-EP452059LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii L-arabinose isomerase (araA) CSB-YP452060LOT
CSB-EP452060LOT
CSB-BP452060LOT
CSB-MP452060LOT
CSB-EP452060LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Glutamate 5-kinase (proB) CSB-YP452061LOT
CSB-EP452061LOT
CSB-BP452061LOT
CSB-MP452061LOT
CSB-EP452061LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Alanine racemase (alr) CSB-YP452062LOT
CSB-EP452062LOT
CSB-BP452062LOT
CSB-MP452062LOT
CSB-EP452062LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Ribose-5-phosphate isomerase A (rpiA) CSB-YP452063LOT
CSB-EP452063LOT
CSB-BP452063LOT
CSB-MP452063LOT
CSB-EP452063LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii UPF0042 nucleotide-binding protein Lcho_3490 (Lcho_3490) CSB-YP452064LOT
CSB-EP452064LOT
CSB-BP452064LOT
CSB-MP452064LOT
CSB-EP452064LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii ATP synthase subunit c (atpE), partial CSB-YP452065LOT1
CSB-EP452065LOT1
CSB-BP452065LOT1
CSB-MP452065LOT1
CSB-EP452065LOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP452066LOT
CSB-EP452066LOT
CSB-BP452066LOT
CSB-MP452066LOT
CSB-EP452066LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP452067LOT
CSB-EP452067LOT
CSB-BP452067LOT
CSB-MP452067LOT
CSB-EP452067LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP452068LOT
CSB-EP452068LOT
CSB-BP452068LOT
CSB-MP452068LOT
CSB-EP452068LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Protein-export protein secB (secB) CSB-YP452069LOT
CSB-EP452069LOT
CSB-BP452069LOT
CSB-MP452069LOT
CSB-EP452069LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Chaperone protein DnaK (dnaK), partial CSB-YP452070LOT
CSB-EP452070LOT
CSB-BP452070LOT
CSB-MP452070LOT
CSB-EP452070LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L7/L12 (rplL) CSB-YP452071LOT
CSB-EP452071LOT
CSB-BP452071LOT
CSB-MP452071LOT
CSB-EP452071LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP452072LOT
CSB-EP452072LOT
CSB-BP452072LOT
CSB-MP452072LOT
CSB-EP452072LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP452073LOT
CSB-EP452073LOT
CSB-BP452073LOT
CSB-MP452073LOT
CSB-EP452073LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Cobyric acid synthase (cobQ), partial CSB-YP452074LOT
CSB-EP452074LOT
CSB-BP452074LOT
CSB-MP452074LOT
CSB-EP452074LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L18 (rplR) CSB-YP452075LOT
CSB-EP452075LOT
CSB-BP452075LOT
CSB-MP452075LOT
CSB-EP452075LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Aspartate carbamoyltransferase (pyrB) CSB-YP452076LOT
CSB-EP452076LOT
CSB-BP452076LOT
CSB-MP452076LOT
CSB-EP452076LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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