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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum ATP synthase subunit c (atpE), partial CSB-YP452857DUG1
CSB-EP452857DUG1
CSB-BP452857DUG1
CSB-MP452857DUG1
CSB-EP452857DUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase gamma chain (atpG) CSB-YP452858DUG
CSB-EP452858DUG
CSB-BP452858DUG
CSB-MP452858DUG
CSB-EP452858DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Methylglyoxal synthase (mgsA) CSB-YP452859DUG
CSB-EP452859DUG
CSB-BP452859DUG
CSB-MP452859DUG
CSB-EP452859DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Xanthine phosphoribosyltransferase (xpt) CSB-YP452860DUG
CSB-EP452860DUG
CSB-BP452860DUG
CSB-MP452860DUG
CSB-EP452860DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP452861DUG
CSB-EP452861DUG
CSB-BP452861DUG
CSB-MP452861DUG
CSB-EP452861DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable rRNA maturation factor (CLL_A0904) CSB-YP452862DUG
CSB-EP452862DUG
CSB-BP452862DUG
CSB-MP452862DUG
CSB-EP452862DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Potassium-transporting ATPase A chain (kdpA), partial CSB-YP452863DUG
CSB-EP452863DUG
CSB-BP452863DUG
CSB-MP452863DUG
CSB-EP452863DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartate--tRNA ligase (aspS), partial CSB-YP452864DUG
CSB-EP452864DUG
CSB-BP452864DUG
CSB-MP452864DUG
CSB-EP452864DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP452865DUG
CSB-EP452865DUG
CSB-BP452865DUG
CSB-MP452865DUG
CSB-EP452865DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0371 protein CLL_A2797 (CLL_A2797), partial CSB-YP452866DUG
CSB-EP452866DUG
CSB-BP452866DUG
CSB-MP452866DUG
CSB-EP452866DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum V-type proton ATPase subunit E (atpE) CSB-YP452867DUG
CSB-EP452867DUG
CSB-BP452867DUG
CSB-MP452867DUG
CSB-EP452867DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP452868DUG
CSB-EP452868DUG
CSB-BP452868DUG
CSB-MP452868DUG
CSB-EP452868DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Enolase (eno) CSB-YP452869DUG
CSB-EP452869DUG
CSB-BP452869DUG
CSB-MP452869DUG
CSB-EP452869DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum D-alanine--D-alanine ligase (ddl) CSB-YP452870DUG
CSB-EP452870DUG
CSB-BP452870DUG
CSB-MP452870DUG
CSB-EP452870DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative sporulation transcription regulator WhiA (whiA) CSB-YP452871DUG
CSB-EP452871DUG
CSB-BP452871DUG
CSB-MP452871DUG
CSB-EP452871DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glycogen synthase (glgA) CSB-YP452872DUG
CSB-EP452872DUG
CSB-BP452872DUG
CSB-MP452872DUG
CSB-EP452872DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L34 (rpmH) CSB-YP452873DUG
CSB-EP452873DUG
CSB-BP452873DUG
CSB-MP452873DUG
CSB-EP452873DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP452874DUG
CSB-EP452874DUG
CSB-BP452874DUG
CSB-MP452874DUG
CSB-EP452874DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum MutS2 protein (mutS2), partial CSB-YP452875DUG
CSB-EP452875DUG
CSB-BP452875DUG
CSB-MP452875DUG
CSB-EP452875DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP452876DUG
CSB-EP452876DUG
CSB-BP452876DUG
CSB-MP452876DUG
CSB-EP452876DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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