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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Alteromonas macleodii Ribonuclease HII (rnhB) CSB-YP451976AZX
CSB-EP451976AZX
CSB-BP451976AZX
CSB-MP451976AZX
CSB-EP451976AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Protein RecA (recA) CSB-YP451977AZX
CSB-EP451977AZX
CSB-BP451977AZX
CSB-MP451977AZX
CSB-EP451977AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Catalase-peroxidase (katG), partial CSB-YP451978AZX
CSB-EP451978AZX
CSB-BP451978AZX
CSB-MP451978AZX
CSB-EP451978AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Protein translocase subunit SecA (secA), partial CSB-YP451979AZX
CSB-EP451979AZX
CSB-BP451979AZX
CSB-MP451979AZX
CSB-EP451979AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Ribosome maturation factor RimP (rimP) CSB-YP451980AZX
CSB-EP451980AZX
CSB-BP451980AZX
CSB-MP451980AZX
CSB-EP451980AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 30S ribosomal protein S15 (rpsO) CSB-YP451981AZX
CSB-EP451981AZX
CSB-BP451981AZX
CSB-MP451981AZX
CSB-EP451981AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L31 (rpmE1) CSB-YP451982AZX
CSB-EP451982AZX
CSB-BP451982AZX
CSB-MP451982AZX
CSB-EP451982AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Trp operon repressor (trpR) CSB-YP451983ENT
CSB-EP451983ENT
CSB-BP451983ENT
CSB-MP451983ENT
CSB-EP451983ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcriptional regulator slyA (slyA) CSB-YP451984ENT
CSB-EP451984ENT
CSB-BP451984ENT
CSB-MP451984ENT
CSB-EP451984ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Vitamin B12 import ATP-binding protein BtuD (btuD) CSB-YP451985ENT
CSB-EP451985ENT
CSB-BP451985ENT
CSB-MP451985ENT
CSB-EP451985ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP451986ENT
CSB-EP451986ENT
CSB-BP451986ENT
CSB-MP451986ENT
CSB-EP451986ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli G/U mismatch-specific DNA glycosylase (mug) CSB-YP451987ENT
CSB-EP451987ENT
CSB-BP451987ENT
CSB-MP451987ENT
CSB-EP451987ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Threonine--tRNA ligase (thrS) CSB-YP451988ENT
CSB-EP451988ENT
CSB-BP451988ENT
CSB-MP451988ENT
CSB-EP451988ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Succinylornithine transaminase (astC) CSB-YP451989ENT
CSB-EP451989ENT
CSB-BP451989ENT
CSB-MP451989ENT
CSB-EP451989ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-tagatose-1,6-bisphosphate aldolase subunit kbaZ (kbaZ) CSB-YP451990ENT
CSB-EP451990ENT
CSB-BP451990ENT
CSB-MP451990ENT
CSB-EP451990ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S15 (rpsO) CSB-YP451991ENT
CSB-EP451991ENT
CSB-BP451991ENT
CSB-MP451991ENT
CSB-EP451991ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP451992ENT
CSB-EP451992ENT
CSB-BP451992ENT
CSB-MP451992ENT
CSB-EP451992ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP451993ENT
CSB-EP451993ENT
CSB-BP451993ENT
CSB-MP451993ENT
CSB-EP451993ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GTPase obg (obg) CSB-YP451994ENT
CSB-EP451994ENT
CSB-BP451994ENT
CSB-MP451994ENT
CSB-EP451994ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcriptional regulator NanR (nanR) CSB-YP451995ENT
CSB-EP451995ENT
CSB-BP451995ENT
CSB-MP451995ENT
CSB-EP451995ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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