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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli DNA-binding protein fis (fis) CSB-YP451996ENT
CSB-EP451996ENT
CSB-BP451996ENT
CSB-MP451996ENT
CSB-EP451996ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S10 (rpsJ) CSB-YP451998FQA
CSB-EP451998FQA
CSB-BP451998FQA
CSB-MP451998FQA
CSB-EP451998FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit O CSB-YP451999FQA
CSB-EP451999FQA
CSB-BP451999FQA
CSB-MP451999FQA
CSB-EP451999FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphomethylpyrimidine synthase (thiC) CSB-YP452000FQA
CSB-EP452000FQA
CSB-BP452000FQA
CSB-MP452000FQA
CSB-EP452000FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L35 (rpmI) CSB-YP452001FQA
CSB-EP452001FQA
CSB-BP452001FQA
CSB-MP452001FQA
CSB-EP452001FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Histidine--tRNA ligase (hisS) CSB-YP452002FQA
CSB-EP452002FQA
CSB-BP452002FQA
CSB-MP452002FQA
CSB-EP452002FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L7/L12 (rplL) CSB-YP452003FQA
CSB-EP452003FQA
CSB-BP452003FQA
CSB-MP452003FQA
CSB-EP452003FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L13 (rplM) CSB-YP452004FQA
CSB-EP452004FQA
CSB-BP452004FQA
CSB-MP452004FQA
CSB-EP452004FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L24 (rplX) CSB-YP452005FQA
CSB-EP452005FQA
CSB-BP452005FQA
CSB-MP452005FQA
CSB-EP452005FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L2 (rplB) CSB-YP452006FQA
CSB-EP452006FQA
CSB-BP452006FQA
CSB-MP452006FQA
CSB-EP452006FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor 4 (lepA), partial CSB-YP452007FQA
CSB-EP452007FQA
CSB-BP452007FQA
CSB-MP452007FQA
CSB-EP452007FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-dehydroquinate synthase (aroB) CSB-YP452008FQA
CSB-EP452008FQA
CSB-BP452008FQA
CSB-MP452008FQA
CSB-EP452008FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Asparagine--tRNA ligase (asnS) CSB-YP452009FQA
CSB-EP452009FQA
CSB-BP452009FQA
CSB-MP452009FQA
CSB-EP452009FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal protein L11 methyltransferase (prmA) CSB-YP452010FQA
CSB-EP452010FQA
CSB-BP452010FQA
CSB-MP452010FQA
CSB-EP452010FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (SYNPCC7002_A1284) CSB-YP452011FQA
CSB-EP452011FQA
CSB-BP452011FQA
CSB-MP452011FQA
CSB-EP452011FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Homoserine kinase (thrB) CSB-YP452012FQA
CSB-EP452012FQA
CSB-BP452012FQA
CSB-MP452012FQA
CSB-EP452012FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UPF0145 protein SYNPCC7002_A1337 (SYNPCC7002_A1337) CSB-YP452013FQA
CSB-EP452013FQA
CSB-BP452013FQA
CSB-MP452013FQA
CSB-EP452013FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. DNA ligase (ligA), partial CSB-YP452014FQA
CSB-EP452014FQA
CSB-BP452014FQA
CSB-MP452014FQA
CSB-EP452014FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UPF0758 protein SYNPCC7002_A0220 (SYNPCC7002_A0220) CSB-YP452015FQA
CSB-EP452015FQA
CSB-BP452015FQA
CSB-MP452015FQA
CSB-EP452015FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP452016FQA
CSB-EP452016FQA
CSB-BP452016FQA
CSB-MP452016FQA
CSB-EP452016FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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