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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia ambifaria ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP452137BXP
CSB-EP452137BXP
CSB-BP452137BXP
CSB-MP452137BXP
CSB-EP452137BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP452138BXP
CSB-EP452138BXP
CSB-BP452138BXP
CSB-MP452138BXP
CSB-EP452138BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Indole-3-glycerol phosphate synthase (trpC) CSB-YP452139BXP
CSB-EP452139BXP
CSB-BP452139BXP
CSB-MP452139BXP
CSB-EP452139BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Cysteine--tRNA ligase (cysS) CSB-YP452140BXP
CSB-EP452140BXP
CSB-BP452140BXP
CSB-MP452140BXP
CSB-EP452140BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP452141BXP
CSB-EP452141BXP
CSB-BP452141BXP
CSB-MP452141BXP
CSB-EP452141BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Glutamate 5-kinase (proB) CSB-YP452142BXP
CSB-EP452142BXP
CSB-BP452142BXP
CSB-MP452142BXP
CSB-EP452142BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Lysine--tRNA ligase (lysS), partial CSB-YP452143BXP
CSB-EP452143BXP
CSB-BP452143BXP
CSB-MP452143BXP
CSB-EP452143BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S9 (rpsI) CSB-YP452144BXP
CSB-EP452144BXP
CSB-BP452144BXP
CSB-MP452144BXP
CSB-EP452144BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP452145BXP
CSB-EP452145BXP
CSB-BP452145BXP
CSB-MP452145BXP
CSB-EP452145BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0246 protein BamMC406_2140 (BamMC406_2140) CSB-YP452146BXP
CSB-EP452146BXP
CSB-BP452146BXP
CSB-MP452146BXP
CSB-EP452146BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP452147BXP
CSB-EP452147BXP
CSB-BP452147BXP
CSB-MP452147BXP
CSB-EP452147BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Protein TolB (tolB) CSB-YP452148BXP
CSB-EP452148BXP
CSB-BP452148BXP
CSB-MP452148BXP
CSB-EP452148BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Probable transcriptional regulatory protein BamMC406_2210 (BamMC406_2210) CSB-YP452149BXP
CSB-EP452149BXP
CSB-BP452149BXP
CSB-MP452149BXP
CSB-EP452149BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Urease subunit beta (ureB) CSB-YP452150BXP
CSB-EP452150BXP
CSB-BP452150BXP
CSB-MP452150BXP
CSB-EP452150BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Ribonuclease PH (rph) CSB-YP452151BXP
CSB-EP452151BXP
CSB-BP452151BXP
CSB-MP452151BXP
CSB-EP452151BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Probable cytosol aminopeptidase (pepA), partial CSB-YP452152BXP
CSB-EP452152BXP
CSB-BP452152BXP
CSB-MP452152BXP
CSB-EP452152BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria D-amino acid dehydrogenase small subunit (dadA) CSB-YP452153BXP
CSB-EP452153BXP
CSB-BP452153BXP
CSB-MP452153BXP
CSB-EP452153BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L28 (rpmB) CSB-YP452154BXP
CSB-EP452154BXP
CSB-BP452154BXP
CSB-MP452154BXP
CSB-EP452154BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP452155BXP
CSB-EP452155BXP
CSB-BP452155BXP
CSB-MP452155BXP
CSB-EP452155BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Kynurenine formamidase (kynB) CSB-YP452156BXP
CSB-EP452156BXP
CSB-BP452156BXP
CSB-MP452156BXP
CSB-EP452156BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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