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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Kocuria rhizophila 50S ribosomal protein L6 (rplF) CSB-YP452337KBO
CSB-EP452337KBO
CSB-BP452337KBO
CSB-MP452337KBO
CSB-EP452337KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Phosphoglucosamine mutase (glmM) CSB-YP452338KBO
CSB-EP452338KBO
CSB-BP452338KBO
CSB-MP452338KBO
CSB-EP452338KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 30S ribosomal protein S6 (rpsF) CSB-YP452339KBO
CSB-EP452339KBO
CSB-BP452339KBO
CSB-MP452339KBO
CSB-EP452339KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP452340KBO
CSB-EP452340KBO
CSB-BP452340KBO
CSB-MP452340KBO
CSB-EP452340KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 30S ribosomal protein S2 (rpsB) CSB-YP452341KBO
CSB-EP452341KBO
CSB-BP452341KBO
CSB-MP452341KBO
CSB-EP452341KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila DNA ligase (ligA), partial CSB-YP452342KBO
CSB-EP452342KBO
CSB-BP452342KBO
CSB-MP452342KBO
CSB-EP452342KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Arginine--tRNA ligase (argS), partial CSB-YP452343KBO
CSB-EP452343KBO
CSB-BP452343KBO
CSB-MP452343KBO
CSB-EP452343KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila UPF0286 protein KRH_09880 (KRH_09880) CSB-YP452344KBO
CSB-EP452344KBO
CSB-BP452344KBO
CSB-MP452344KBO
CSB-EP452344KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S8 (rpsH) CSB-YP452345MVS
CSB-EP452345MVS
CSB-BP452345MVS
CSB-MP452345MVS
CSB-EP452345MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_1058 (MMAR_1058) CSB-YP452346MVS
CSB-EP452346MVS
CSB-BP452346MVS
CSB-MP452346MVS
CSB-EP452346MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S13 (rpsM) CSB-YP452347MVS
CSB-EP452347MVS
CSB-BP452347MVS
CSB-MP452347MVS
CSB-EP452347MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP452348MVS
CSB-EP452348MVS
CSB-BP452348MVS
CSB-MP452348MVS
CSB-EP452348MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP452349MVS
CSB-EP452349MVS
CSB-BP452349MVS
CSB-MP452349MVS
CSB-EP452349MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0060 membrane protein MMAR_2961 (MMAR_2961), partial CSB-YP452350MVS1
CSB-EP452350MVS1
CSB-BP452350MVS1
CSB-MP452350MVS1
CSB-EP452350MVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Proteasome-associated ATPase (mpa), partial CSB-YP452351MVS
CSB-EP452351MVS
CSB-BP452351MVS
CSB-MP452351MVS
CSB-EP452351MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP452352MVS1
CSB-EP452352MVS1
CSB-BP452352MVS1
CSB-MP452352MVS1
CSB-EP452352MVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Acetaldehyde dehydrogenase 1 (mhpF) CSB-YP452353MVS
CSB-EP452353MVS
CSB-BP452353MVS
CSB-MP452353MVS
CSB-EP452353MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP452354MVS
CSB-EP452354MVS
CSB-BP452354MVS
CSB-MP452354MVS
CSB-EP452354MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP452355MVS
CSB-EP452355MVS
CSB-BP452355MVS
CSB-MP452355MVS
CSB-EP452355MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Octanoyltransferase (lipB) CSB-YP452356MVS
CSB-EP452356MVS
CSB-BP452356MVS
CSB-MP452356MVS
CSB-EP452356MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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