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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia ambifaria ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP454078BXP
CSB-EP454078BXP
CSB-BP454078BXP
CSB-MP454078BXP
CSB-EP454078BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L1 (rplA) CSB-YP454079BXP
CSB-EP454079BXP
CSB-BP454079BXP
CSB-MP454079BXP
CSB-EP454079BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L23 (rplW) CSB-YP454080BXP
CSB-EP454080BXP
CSB-BP454080BXP
CSB-MP454080BXP
CSB-EP454080BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0133 protein BamMC406_1737 (BamMC406_1737) CSB-YP454081BXP
CSB-EP454081BXP
CSB-BP454081BXP
CSB-MP454081BXP
CSB-EP454081BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0061 protein BamMC406_1826 (BamMC406_1826), partial CSB-YP454082BXP
CSB-EP454082BXP
CSB-BP454082BXP
CSB-MP454082BXP
CSB-EP454082BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S8 (rpsH) CSB-YP454083BXP
CSB-EP454083BXP
CSB-BP454083BXP
CSB-MP454083BXP
CSB-EP454083BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 30S ribosomal protein S4 (rpsD) CSB-YP454084BXP
CSB-EP454084BXP
CSB-BP454084BXP
CSB-MP454084BXP
CSB-EP454084BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP454085BXP
CSB-EP454085BXP
CSB-BP454085BXP
CSB-MP454085BXP
CSB-EP454085BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Trigger factor (tig) CSB-YP454086BXP
CSB-EP454086BXP
CSB-BP454086BXP
CSB-MP454086BXP
CSB-EP454086BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP454087BXP
CSB-EP454087BXP
CSB-BP454087BXP
CSB-MP454087BXP
CSB-EP454087BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP454088BXP
CSB-EP454088BXP
CSB-BP454088BXP
CSB-MP454088BXP
CSB-EP454088BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP454089BXP
CSB-EP454089BXP
CSB-BP454089BXP
CSB-MP454089BXP
CSB-EP454089BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0289 protein BamMC406_0502 (BamMC406_0502) CSB-YP454090BXP
CSB-EP454090BXP
CSB-BP454090BXP
CSB-MP454090BXP
CSB-EP454090BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP454091BXP1
CSB-EP454091BXP1
CSB-BP454091BXP1
CSB-MP454091BXP1
CSB-EP454091BXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP454092BXP
CSB-EP454092BXP
CSB-BP454092BXP
CSB-MP454092BXP
CSB-EP454092BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Heat-inducible transcription repressor HrcA (hrcA) CSB-YP454093BXP
CSB-EP454093BXP
CSB-BP454093BXP
CSB-MP454093BXP
CSB-EP454093BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP454094BXP
CSB-EP454094BXP
CSB-BP454094BXP
CSB-MP454094BXP
CSB-EP454094BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0301 protein BamMC406_0754 (BamMC406_0754) CSB-YP454095BXP
CSB-EP454095BXP
CSB-BP454095BXP
CSB-MP454095BXP
CSB-EP454095BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0176 protein BamMC406_2233 (BamMC406_2233) CSB-YP454096BXP
CSB-EP454096BXP
CSB-BP454096BXP
CSB-MP454096BXP
CSB-EP454096BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Protein CrcB homolog (crcB), partial CSB-YP454097BXP1
CSB-EP454097BXP1
CSB-BP454097BXP1
CSB-MP454097BXP1
CSB-EP454097BXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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