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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB ATP synthase subunit c (atpE), partial CSB-YP452617YAW1
CSB-EP452617YAW1
CSB-BP452617YAW1
CSB-MP452617YAW1
CSB-EP452617YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0250 protein YPTS_1150 (YPTS_1150) CSB-YP452618YAW
CSB-EP452618YAW
CSB-BP452618YAW
CSB-MP452618YAW
CSB-EP452618YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Probable rRNA maturation factor (YPTS_1168) CSB-YP452619YAW
CSB-EP452619YAW
CSB-BP452619YAW
CSB-MP452619YAW
CSB-EP452619YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP452620YAW
CSB-EP452620YAW
CSB-BP452620YAW
CSB-MP452620YAW
CSB-EP452620YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UvrABC system protein B (uvrB), partial CSB-YP452621YAW
CSB-EP452621YAW
CSB-BP452621YAW
CSB-MP452621YAW
CSB-EP452621YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Probable manganese transport protein MntH (mntH), partial CSB-YP452622YAW
CSB-EP452622YAW
CSB-BP452622YAW
CSB-MP452622YAW
CSB-EP452622YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP452623YAW
CSB-EP452623YAW
CSB-BP452623YAW
CSB-MP452623YAW
CSB-EP452623YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Multidrug resistance protein MdtA (mdtA) CSB-YP452624YAW
CSB-EP452624YAW
CSB-BP452624YAW
CSB-MP452624YAW
CSB-EP452624YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Iron-binding protein IscA (iscA) CSB-YP452625YAW
CSB-EP452625YAW
CSB-BP452625YAW
CSB-MP452625YAW
CSB-EP452625YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Uncharacterized MFS-type transporter YPTS_1506 (YPTS_1506), partial CSB-YP452626YAW
CSB-EP452626YAW
CSB-BP452626YAW
CSB-MP452626YAW
CSB-EP452626YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB DNA repair protein recO (recO) CSB-YP452627YAW
CSB-EP452627YAW
CSB-BP452627YAW
CSB-MP452627YAW
CSB-EP452627YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Urease subunit alpha (ureC), partial CSB-YP452628YAW
CSB-EP452628YAW
CSB-BP452628YAW
CSB-MP452628YAW
CSB-EP452628YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Chaperone protein DnaK (dnaK), partial CSB-YP452629EKE
CSB-EP452629EKE
CSB-BP452629EKE
CSB-MP452629EKE
CSB-EP452629EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Histidine--tRNA ligase (hisS) CSB-YP452630EKE
CSB-EP452630EKE
CSB-BP452630EKE
CSB-MP452630EKE
CSB-EP452630EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Protein translocase subunit SecF (secF), partial CSB-YP452631EKE1
CSB-EP452631EKE1
CSB-BP452631EKE1
CSB-MP452631EKE1
CSB-EP452631EKE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Maf-like protein Emin_0602 (Emin_0602) CSB-YP452632EKE
CSB-EP452632EKE
CSB-BP452632EKE
CSB-MP452632EKE
CSB-EP452632EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Tryptophan synthase beta chain (trpB) CSB-YP452633EKE
CSB-EP452633EKE
CSB-BP452633EKE
CSB-MP452633EKE
CSB-EP452633EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP452634EKE
CSB-EP452634EKE
CSB-BP452634EKE
CSB-MP452634EKE
CSB-EP452634EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 30S ribosomal protein S15 (rpsO) CSB-YP452635EKE
CSB-EP452635EKE
CSB-BP452635EKE
CSB-MP452635EKE
CSB-EP452635EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP452636EKE
CSB-EP452636EKE
CSB-BP452636EKE
CSB-MP452636EKE
CSB-EP452636EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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