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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Shikimate dehydrogenase (aroE) CSB-YP452597YAW
CSB-EP452597YAW
CSB-BP452597YAW
CSB-MP452597YAW
CSB-EP452597YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP452598YAW
CSB-EP452598YAW
CSB-BP452598YAW
CSB-MP452598YAW
CSB-EP452598YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L15 (rplO) CSB-YP452599YAW
CSB-EP452599YAW
CSB-BP452599YAW
CSB-MP452599YAW
CSB-EP452599YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP452600YAW
CSB-EP452600YAW
CSB-BP452600YAW
CSB-MP452600YAW
CSB-EP452600YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB HTH-type transcriptional repressor PurR (purR) CSB-YP452601YAW
CSB-EP452601YAW
CSB-BP452601YAW
CSB-MP452601YAW
CSB-EP452601YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP452602YAW1
CSB-EP452602YAW1
CSB-BP452602YAW1
CSB-MP452602YAW1
CSB-EP452602YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L22 (rplV) CSB-YP452603YAW
CSB-EP452603YAW
CSB-BP452603YAW
CSB-MP452603YAW
CSB-EP452603YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 30S ribosomal protein S7 (rpsG) CSB-YP452604YAW
CSB-EP452604YAW
CSB-BP452604YAW
CSB-MP452604YAW
CSB-EP452604YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein tsgA homolog (tsgA), partial CSB-YP452605YAW
CSB-EP452605YAW
CSB-BP452605YAW
CSB-MP452605YAW
CSB-EP452605YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Rhomboid protease glpG (glpG), partial CSB-YP452606YAW1
CSB-EP452606YAW1
CSB-BP452606YAW1
CSB-MP452606YAW1
CSB-EP452606YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP452607YAW
CSB-EP452607YAW
CSB-BP452607YAW
CSB-MP452607YAW
CSB-EP452607YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Gamma-glutamyl phosphate reductase (proA) CSB-YP452608YAW
CSB-EP452608YAW
CSB-BP452608YAW
CSB-MP452608YAW
CSB-EP452608YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Recombination-associated protein rdgC (rdgC) CSB-YP452609YAW
CSB-EP452609YAW
CSB-BP452609YAW
CSB-MP452609YAW
CSB-EP452609YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP452610YAW
CSB-EP452610YAW
CSB-BP452610YAW
CSB-MP452610YAW
CSB-EP452610YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB High frequency lysogenization protein HflD homolog (hflD) CSB-YP452611YAW
CSB-EP452611YAW
CSB-BP452611YAW
CSB-MP452611YAW
CSB-EP452611YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Dihydroorotase (pyrC) CSB-YP452612YAW
CSB-EP452612YAW
CSB-BP452612YAW
CSB-MP452612YAW
CSB-EP452612YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP452613YAW
CSB-EP452613YAW
CSB-BP452613YAW
CSB-MP452613YAW
CSB-EP452613YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Low affinity potassium transport system protein kup (kup), partial CSB-YP452614YAW
CSB-EP452614YAW
CSB-BP452614YAW
CSB-MP452614YAW
CSB-EP452614YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP452615YAW1
CSB-EP452615YAW1
CSB-BP452615YAW1
CSB-MP452615YAW1
CSB-EP452615YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0304 protein YPTS_2689 (YPTS_2689) CSB-YP452616YAW
CSB-EP452616YAW
CSB-BP452616YAW
CSB-MP452616YAW
CSB-EP452616YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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