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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia phymatum Urease subunit beta (ureB) CSB-YP452537BXT
CSB-EP452537BXT
CSB-BP452537BXT
CSB-MP452537BXT
CSB-EP452537BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L32 (rpmF) CSB-YP452538BXT
CSB-EP452538BXT
CSB-BP452538BXT
CSB-MP452538BXT
CSB-EP452538BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP452539BXT
CSB-EP452539BXT
CSB-BP452539BXT
CSB-MP452539BXT
CSB-EP452539BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Chaperone protein DnaK (dnaK), partial CSB-YP452540BXT
CSB-EP452540BXT
CSB-BP452540BXT
CSB-MP452540BXT
CSB-EP452540BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Trigger factor (tig) CSB-YP452541BXT
CSB-EP452541BXT
CSB-BP452541BXT
CSB-MP452541BXT
CSB-EP452541BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S9 (rpsI) CSB-YP452542BXT
CSB-EP452542BXT
CSB-BP452542BXT
CSB-MP452542BXT
CSB-EP452542BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ribosomal protein L11 methyltransferase (prmA) CSB-YP452543BXT
CSB-EP452543BXT
CSB-BP452543BXT
CSB-MP452543BXT
CSB-EP452543BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP452544BXT
CSB-EP452544BXT
CSB-BP452544BXT
CSB-MP452544BXT
CSB-EP452544BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP452545BXT
CSB-EP452545BXT
CSB-BP452545BXT
CSB-MP452545BXT
CSB-EP452545BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L17 (rplQ) CSB-YP452546BXT
CSB-EP452546BXT
CSB-BP452546BXT
CSB-MP452546BXT
CSB-EP452546BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L6 (rplF) CSB-YP452547BXT
CSB-EP452547BXT
CSB-BP452547BXT
CSB-MP452547BXT
CSB-EP452547BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Protein RnfH (rnfH) CSB-YP452548BXT
CSB-EP452548BXT
CSB-BP452548BXT
CSB-MP452548BXT
CSB-EP452548BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP452549BXT
CSB-EP452549BXT
CSB-BP452549BXT
CSB-MP452549BXT
CSB-EP452549BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP452550BXT
CSB-EP452550BXT
CSB-BP452550BXT
CSB-MP452550BXT
CSB-EP452550BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP452551BXT
CSB-EP452551BXT
CSB-BP452551BXT
CSB-MP452551BXT
CSB-EP452551BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Bifunctional protein GlmU (glmU) CSB-YP452552BXT
CSB-EP452552BXT
CSB-BP452552BXT
CSB-MP452552BXT
CSB-EP452552BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Protein hfq (hfq) CSB-YP452553BXT
CSB-EP452553BXT
CSB-BP452553BXT
CSB-MP452553BXT
CSB-EP452553BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Enolase (eno) CSB-YP452554BXT
CSB-EP452554BXT
CSB-BP452554BXT
CSB-MP452554BXT
CSB-EP452554BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum ATP synthase gamma chain (atpG) CSB-YP452555BXT
CSB-EP452555BXT
CSB-BP452555BXT
CSB-MP452555BXT
CSB-EP452555BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L35 (rpmI) CSB-YP452556BXT
CSB-EP452556BXT
CSB-BP452556BXT
CSB-MP452556BXT
CSB-EP452556BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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