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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Stenotrophomonas maltophilia Sec-independent protein translocase protein TatA (tatA), partial CSB-YP454218FLX
CSB-EP454218FLX
CSB-BP454218FLX
CSB-MP454218FLX
CSB-EP454218FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP454219FLX
CSB-EP454219FLX
CSB-BP454219FLX
CSB-MP454219FLX
CSB-EP454219FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Histidine biosynthesis bifunctional protein hisB (hisB) CSB-YP454220FLX
CSB-EP454220FLX
CSB-BP454220FLX
CSB-MP454220FLX
CSB-EP454220FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia DNA repair protein recO (recO) CSB-YP454221FLX
CSB-EP454221FLX
CSB-BP454221FLX
CSB-MP454221FLX
CSB-EP454221FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UPF0761 membrane protein Smlt0865 (Smlt0865), partial CSB-YP454222FLX1
CSB-EP454222FLX1
CSB-BP454222FLX1
CSB-MP454222FLX1
CSB-EP454222FLX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP454223FLX
CSB-EP454223FLX
CSB-BP454223FLX
CSB-MP454223FLX
CSB-EP454223FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L2 (rplB) CSB-YP454224FLX
CSB-EP454224FLX
CSB-BP454224FLX
CSB-MP454224FLX
CSB-EP454224FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP454225FLX
CSB-EP454225FLX
CSB-BP454225FLX
CSB-MP454225FLX
CSB-EP454225FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S5 (rpsE) CSB-YP454226FLX
CSB-EP454226FLX
CSB-BP454226FLX
CSB-MP454226FLX
CSB-EP454226FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Peptidyl-Asp metalloendopeptidase (Smlt0970) CSB-YP454227FLX
CSB-EP454227FLX
CSB-BP454227FLX
CSB-MP454227FLX
CSB-EP454227FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP454228FLX
CSB-EP454228FLX
CSB-BP454228FLX
CSB-MP454228FLX
CSB-EP454228FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 3-deoxy-D-manno-octulosonic acid kinase (kdkA) CSB-YP454229FLX
CSB-EP454229FLX
CSB-BP454229FLX
CSB-MP454229FLX
CSB-EP454229FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP454230FLX
CSB-EP454230FLX
CSB-BP454230FLX
CSB-MP454230FLX
CSB-EP454230FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L31 type B (rpmE2) CSB-YP454231FLX
CSB-EP454231FLX
CSB-BP454231FLX
CSB-MP454231FLX
CSB-EP454231FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP454232FLX
CSB-EP454232FLX
CSB-BP454232FLX
CSB-MP454232FLX
CSB-EP454232FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP454233FLX
CSB-EP454233FLX
CSB-BP454233FLX
CSB-MP454233FLX
CSB-EP454233FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Probable GTP-binding protein EngB (engB) CSB-YP454234FLX
CSB-EP454234FLX
CSB-BP454234FLX
CSB-MP454234FLX
CSB-EP454234FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S16 (rpsP) CSB-YP454235FLX
CSB-EP454235FLX
CSB-BP454235FLX
CSB-MP454235FLX
CSB-EP454235FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UPF0250 protein Smlt4048 (Smlt4048) CSB-YP454236FLX
CSB-EP454236FLX
CSB-BP454236FLX
CSB-MP454236FLX
CSB-EP454236FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii Restriction of telomere capping protein 4 (RTC4) CSB-YP454237ZBK
CSB-EP454237ZBK
CSB-BP454237ZBK
CSB-MP454237ZBK
CSB-EP454237ZBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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