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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Elusimicrobium minutum UPF0059 membrane protein Emin_1326 (Emin_1326), partial CSB-YP452637EKE1
CSB-EP452637EKE1
CSB-BP452637EKE1
CSB-MP452637EKE1
CSB-EP452637EKE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 30S ribosomal protein S13 (rpsM) CSB-YP452638EKE
CSB-EP452638EKE
CSB-BP452638EKE
CSB-MP452638EKE
CSB-EP452638EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 30S ribosomal protein S3 (rpsC) CSB-YP452639EKE
CSB-EP452639EKE
CSB-BP452639EKE
CSB-MP452639EKE
CSB-EP452639EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP452640EKE
CSB-EP452640EKE
CSB-BP452640EKE
CSB-MP452640EKE
CSB-EP452640EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Serine--tRNA ligase (serS) CSB-YP452641EKE
CSB-EP452641EKE
CSB-BP452641EKE
CSB-MP452641EKE
CSB-EP452641EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum ATP synthase gamma chain (atpG) CSB-YP452642EKE
CSB-EP452642EKE
CSB-BP452642EKE
CSB-MP452642EKE
CSB-EP452642EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus striatus Delta-conotoxin-like S6.8 CSB-YP452643DWI
CSB-EP452643DWI
CSB-BP452643DWI
CSB-MP452643DWI
CSB-EP452643DWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus marmoreus Conomarphin CSB-YP452644DWD
CSB-EP452644DWD
CSB-BP452644DWD
CSB-MP452644DWD
CSB-EP452644DWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP452645GHI1
CSB-EP452645GHI1
CSB-BP452645GHI1
CSB-MP452645GHI1
CSB-EP452645GHI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica Photosystem II reaction center protein L (psbL), partial CSB-YP452646GHI
CSB-EP452646GHI
CSB-BP452646GHI
CSB-MP452646GHI
CSB-EP452646GHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP452647GHI
CSB-EP452647GHI
CSB-BP452647GHI
CSB-MP452647GHI
CSB-EP452647GHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guizotia abyssinica NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic, partial CSB-YP452648GHI1
CSB-EP452648GHI1
CSB-BP452648GHI1
CSB-MP452648GHI1
CSB-EP452648GHI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemaphysalis longicornis Longicornsin (sGDLP) CSB-YP452649HSV
CSB-EP452649HSV
CSB-BP452649HSV
CSB-MP452649HSV
CSB-EP452649HSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens Sensory neuron membrane protein 2 (snmp2), partial CSB-YP452650HVF
CSB-EP452650HVF
CSB-BP452650HVF
CSB-MP452650HVF
CSB-EP452650HVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Chaperone protein htpG (htpG), partial CSB-YP452651EXZ
CSB-EP452651EXZ
CSB-BP452651EXZ
CSB-MP452651EXZ
CSB-EP452651EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Thiazole synthase (thiG) CSB-YP452652EXZ
CSB-EP452652EXZ
CSB-BP452652EXZ
CSB-MP452652EXZ
CSB-EP452652EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP452653EXZ
CSB-EP452653EXZ
CSB-BP452653EXZ
CSB-MP452653EXZ
CSB-EP452653EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Probable transaldolase (tal) CSB-YP452654EXZ
CSB-EP452654EXZ
CSB-BP452654EXZ
CSB-MP452654EXZ
CSB-EP452654EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Lipoprotein signal peptidase (lspA), partial CSB-YP452655EXZ1
CSB-EP452655EXZ1
CSB-BP452655EXZ1
CSB-MP452655EXZ1
CSB-EP452655EXZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP452656EXZ
CSB-EP452656EXZ
CSB-BP452656EXZ
CSB-MP452656EXZ
CSB-EP452656EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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