Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia phytofirmans Putative membrane protein insertion efficiency factor (Bphyt_3987) CSB-YP452837BXU
CSB-EP452837BXU
CSB-BP452837BXU
CSB-MP452837BXU
CSB-EP452837BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0317 protein Bphyt_4813 (Bphyt_4813) CSB-YP452838BXU
CSB-EP452838BXU
CSB-BP452838BXU
CSB-MP452838BXU
CSB-EP452838BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Putative 3-methyladenine DNA glycosylase (Bphyt_4972) CSB-YP452839BXU
CSB-EP452839BXU
CSB-BP452839BXU
CSB-MP452839BXU
CSB-EP452839BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Formimidoylglutamase (hutG) CSB-YP452840BXU
CSB-EP452840BXU
CSB-BP452840BXU
CSB-MP452840BXU
CSB-EP452840BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Mannonate dehydratase (uxuA) CSB-YP452841BXU
CSB-EP452841BXU
CSB-BP452841BXU
CSB-MP452841BXU
CSB-EP452841BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Gamma-glutamyl phosphate reductase (proA) CSB-YP452842DUG
CSB-EP452842DUG
CSB-BP452842DUG
CSB-MP452842DUG
CSB-EP452842DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP452843DUG
CSB-EP452843DUG
CSB-BP452843DUG
CSB-MP452843DUG
CSB-EP452843DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional protein GlmU (glmU) CSB-YP452844DUG
CSB-EP452844DUG
CSB-BP452844DUG
CSB-MP452844DUG
CSB-EP452844DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Protein hfq (hfq) CSB-YP452845DUG
CSB-EP452845DUG
CSB-BP452845DUG
CSB-MP452845DUG
CSB-EP452845DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative ATP:guanido phosphotransferase CLL_A0206 (CLL_A0206) CSB-YP452846DUG
CSB-EP452846DUG
CSB-BP452846DUG
CSB-MP452846DUG
CSB-EP452846DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L1 (rplA) CSB-YP452847DUG
CSB-EP452847DUG
CSB-BP452847DUG
CSB-MP452847DUG
CSB-EP452847DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S3 (rpsC) CSB-YP452848DUG
CSB-EP452848DUG
CSB-BP452848DUG
CSB-MP452848DUG
CSB-EP452848DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S8 (rpsH) CSB-YP452849DUG
CSB-EP452849DUG
CSB-BP452849DUG
CSB-MP452849DUG
CSB-EP452849DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L36 (rpmJ) CSB-YP452850DUG
CSB-EP452850DUG
CSB-BP452850DUG
CSB-MP452850DUG
CSB-EP452850DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L17 (rplQ) CSB-YP452851DUG
CSB-EP452851DUG
CSB-BP452851DUG
CSB-MP452851DUG
CSB-EP452851DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ketol-acid reductoisomerase (ilvC) CSB-YP452852DUG
CSB-EP452852DUG
CSB-BP452852DUG
CSB-MP452852DUG
CSB-EP452852DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP452853DUG
CSB-EP452853DUG
CSB-BP452853DUG
CSB-MP452853DUG
CSB-EP452853DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphate acyltransferase (plsX) CSB-YP452854DUG
CSB-EP452854DUG
CSB-BP452854DUG
CSB-MP452854DUG
CSB-EP452854DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP452855DUG
CSB-EP452855DUG
CSB-BP452855DUG
CSB-MP452855DUG
CSB-EP452855DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S15 (rpsO) CSB-YP452856DUG
CSB-EP452856DUG
CSB-BP452856DUG
CSB-MP452856DUG
CSB-EP452856DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>