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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP452797XBC
CSB-EP452797XBC
CSB-BP452797XBC
CSB-MP452797XBC
CSB-EP452797XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP452798XBC
CSB-EP452798XBC
CSB-BP452798XBC
CSB-MP452798XBC
CSB-EP452798XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP452799XBC
CSB-EP452799XBC
CSB-BP452799XBC
CSB-MP452799XBC
CSB-EP452799XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP452800XBC
CSB-EP452800XBC
CSB-BP452800XBC
CSB-MP452800XBC
CSB-EP452800XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0250 protein Bphyt_0500 (Bphyt_0500) CSB-YP452801BXU
CSB-EP452801BXU
CSB-BP452801BXU
CSB-MP452801BXU
CSB-EP452801BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP452802BXU
CSB-EP452802BXU
CSB-BP452802BXU
CSB-MP452802BXU
CSB-EP452802BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP452803BXU
CSB-EP452803BXU
CSB-BP452803BXU
CSB-MP452803BXU
CSB-EP452803BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Chaperone protein DnaK (dnaK), partial CSB-YP452804BXU
CSB-EP452804BXU
CSB-BP452804BXU
CSB-MP452804BXU
CSB-EP452804BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans DNA mismatch repair protein MutS (mutS), partial CSB-YP452805BXU
CSB-EP452805BXU
CSB-BP452805BXU
CSB-MP452805BXU
CSB-EP452805BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP452806BXU
CSB-EP452806BXU
CSB-BP452806BXU
CSB-MP452806BXU
CSB-EP452806BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP452807BXU
CSB-EP452807BXU
CSB-BP452807BXU
CSB-MP452807BXU
CSB-EP452807BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glycerol kinase (glpK) CSB-YP452808BXU
CSB-EP452808BXU
CSB-BP452808BXU
CSB-MP452808BXU
CSB-EP452808BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP452809BXU
CSB-EP452809BXU
CSB-BP452809BXU
CSB-MP452809BXU
CSB-EP452809BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP452810BXU1
CSB-EP452810BXU1
CSB-BP452810BXU1
CSB-MP452810BXU1
CSB-EP452810BXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Protein translocase subunit SecA (secA), partial CSB-YP452811BXU
CSB-EP452811BXU
CSB-BP452811BXU
CSB-MP452811BXU
CSB-EP452811BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Indole-3-glycerol phosphate synthase (trpC) CSB-YP452812BXU
CSB-EP452812BXU
CSB-BP452812BXU
CSB-MP452812BXU
CSB-EP452812BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP452813BXU
CSB-EP452813BXU
CSB-BP452813BXU
CSB-MP452813BXU
CSB-EP452813BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Threonine--tRNA ligase (thrS), partial CSB-YP452814BXU
CSB-EP452814BXU
CSB-BP452814BXU
CSB-MP452814BXU
CSB-EP452814BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Elongation factor 4 (lepA), partial CSB-YP452815BXU
CSB-EP452815BXU
CSB-BP452815BXU
CSB-MP452815BXU
CSB-EP452815BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0301 protein Bphyt_0868 (Bphyt_0868) CSB-YP452816BXU
CSB-EP452816BXU
CSB-BP452816BXU
CSB-MP452816BXU
CSB-EP452816BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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