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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Natranaerobius thermophilus UPF0296 protein Nther_1328 (Nther_1328) CSB-YP454158NFO
CSB-EP454158NFO
CSB-BP454158NFO
CSB-MP454158NFO
CSB-EP454158NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 30S ribosomal protein S16 (rpsP) CSB-YP454159NFO
CSB-EP454159NFO
CSB-BP454159NFO
CSB-MP454159NFO
CSB-EP454159NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus DNA replication and repair protein recF (recF) CSB-YP454160NFO
CSB-EP454160NFO
CSB-BP454160NFO
CSB-MP454160NFO
CSB-EP454160NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 30S ribosomal protein S15 (rpsO) CSB-YP454161NFO
CSB-EP454161NFO
CSB-BP454161NFO
CSB-MP454161NFO
CSB-EP454161NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus ATP synthase subunit c (atpE), partial CSB-YP454162NFO1
CSB-EP454162NFO1
CSB-BP454162NFO1
CSB-MP454162NFO1
CSB-EP454162NFO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP454163NFO
CSB-EP454163NFO
CSB-BP454163NFO
CSB-MP454163NFO
CSB-EP454163NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus tRNA modification GTPase MnmE (mnmE) CSB-YP454164NFO
CSB-EP454164NFO
CSB-BP454164NFO
CSB-MP454164NFO
CSB-EP454164NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 50S ribosomal protein L33 (rpmG) CSB-YP454165NFO
CSB-EP454165NFO
CSB-BP454165NFO
CSB-MP454165NFO
CSB-EP454165NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 30S ribosomal protein S3 (rpsC) CSB-YP454166NFO
CSB-EP454166NFO
CSB-BP454166NFO
CSB-MP454166NFO
CSB-EP454166NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 30S ribosomal protein S5 (rpsE) CSB-YP454167NFO
CSB-EP454167NFO
CSB-BP454167NFO
CSB-MP454167NFO
CSB-EP454167NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 30S ribosomal protein S11 (rpsK) CSB-YP454168NFO
CSB-EP454168NFO
CSB-BP454168NFO
CSB-MP454168NFO
CSB-EP454168NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Elongation factor P (efp) CSB-YP454169NFO
CSB-EP454169NFO
CSB-BP454169NFO
CSB-MP454169NFO
CSB-EP454169NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus UPF0291 protein Nther_1806 (Nther_1806) CSB-YP454170NFO
CSB-EP454170NFO
CSB-BP454170NFO
CSB-MP454170NFO
CSB-EP454170NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Redox-sensing transcriptional repressor rex (rex) CSB-YP454171NFO
CSB-EP454171NFO
CSB-BP454171NFO
CSB-MP454171NFO
CSB-EP454171NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Non-canonical purine NTP pyrophosphatase (Nther_1950) CSB-YP454172NFO
CSB-EP454172NFO
CSB-BP454172NFO
CSB-MP454172NFO
CSB-EP454172NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus UPF0042 nucleotide-binding protein Nther_2024 (Nther_2024) CSB-YP454173NFO
CSB-EP454173NFO
CSB-BP454173NFO
CSB-MP454173NFO
CSB-EP454173NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Homoserine kinase (thrB) CSB-YP454174NFO
CSB-EP454174NFO
CSB-BP454174NFO
CSB-MP454174NFO
CSB-EP454174NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Long chronological lifespan protein 2 (LCL2) CSB-YP454175EXJ
CSB-EP454175EXJ
CSB-BP454175EXJ
CSB-MP454175EXJ
CSB-EP454175EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Probable Xaa-Pro aminopeptidase PEPP (PEPP) CSB-YP454176EXJ
CSB-EP454176EXJ
CSB-BP454176EXJ
CSB-MP454176EXJ
CSB-EP454176EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Pyrrolidone-carboxylate peptidase (pcp) CSB-YP454177DZS
CSB-EP454177DZS
CSB-BP454177DZS
CSB-MP454177DZS
CSB-EP454177DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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