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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phenylobacterium zucineum Thiazole synthase (thiG) CSB-YP455819EUP
CSB-EP455819EUP
CSB-BP455819EUP
CSB-MP455819EUP
CSB-EP455819EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP455820EUP
CSB-EP455820EUP
CSB-BP455820EUP
CSB-MP455820EUP
CSB-EP455820EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 5'-nucleotidase surE (surE) CSB-YP455821EUP
CSB-EP455821EUP
CSB-BP455821EUP
CSB-MP455821EUP
CSB-EP455821EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UPF0133 protein PHZ_c0369 (PHZ_c0369) CSB-YP455822EUP
CSB-EP455822EUP
CSB-BP455822EUP
CSB-MP455822EUP
CSB-EP455822EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Adenylosuccinate synthetase (purA) CSB-YP455823EUP
CSB-EP455823EUP
CSB-BP455823EUP
CSB-MP455823EUP
CSB-EP455823EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UPF0246 protein PHZ_c0561 (PHZ_c0561) CSB-YP455824EUP
CSB-EP455824EUP
CSB-BP455824EUP
CSB-MP455824EUP
CSB-EP455824EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP455825EUP
CSB-EP455825EUP
CSB-BP455825EUP
CSB-MP455825EUP
CSB-EP455825EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP455826EUP
CSB-EP455826EUP
CSB-BP455826EUP
CSB-MP455826EUP
CSB-EP455826EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 3-dehydroquinate synthase (aroB) CSB-YP455827EUP
CSB-EP455827EUP
CSB-BP455827EUP
CSB-MP455827EUP
CSB-EP455827EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP455828EUP
CSB-EP455828EUP
CSB-BP455828EUP
CSB-MP455828EUP
CSB-EP455828EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 30S ribosomal protein S4 (rpsD) CSB-YP455829EUP
CSB-EP455829EUP
CSB-BP455829EUP
CSB-MP455829EUP
CSB-EP455829EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L19 (rplS) CSB-YP455830NGB
CSB-EP455830NGB
CSB-BP455830NGB
CSB-MP455830NGB
CSB-EP455830NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Sec-independent protein translocase protein TatA (tatA), partial CSB-YP455831NGB
CSB-EP455831NGB
CSB-BP455831NGB
CSB-MP455831NGB
CSB-EP455831NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 33 kDa chaperonin (hslO) CSB-YP455832NGB
CSB-EP455832NGB
CSB-BP455832NGB
CSB-MP455832NGB
CSB-EP455832NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Membrane protein insertase YidC (yidC), partial CSB-YP455833NGB1
CSB-EP455833NGB1
CSB-BP455833NGB1
CSB-MP455833NGB1
CSB-EP455833NGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Putative phosphotransferase NGK_0333 (NGK_0333) CSB-YP455834NGB
CSB-EP455834NGB
CSB-BP455834NGB
CSB-MP455834NGB
CSB-EP455834NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP455835NGB
CSB-EP455835NGB
CSB-BP455835NGB
CSB-MP455835NGB
CSB-EP455835NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L20 (rplT) CSB-YP455836NGB
CSB-EP455836NGB
CSB-BP455836NGB
CSB-MP455836NGB
CSB-EP455836NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP455837NGB
CSB-EP455837NGB
CSB-BP455837NGB
CSB-MP455837NGB
CSB-EP455837NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP455838NGB
CSB-EP455838NGB
CSB-BP455838NGB
CSB-MP455838NGB
CSB-EP455838NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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