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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum S-adenosylmethionine synthase (metK) CSB-YP455018DUF
CSB-EP455018DUF
CSB-BP455018DUF
CSB-MP455018DUF
CSB-EP455018DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Maf-like protein CLH_0546 (CLH_0546) CSB-YP455019DUF
CSB-EP455019DUF
CSB-BP455019DUF
CSB-MP455019DUF
CSB-EP455019DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Selenide, water dikinase (selD) CSB-YP455020DUF
CSB-EP455020DUF
CSB-BP455020DUF
CSB-MP455020DUF
CSB-EP455020DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP455021DUF
CSB-EP455021DUF
CSB-BP455021DUF
CSB-MP455021DUF
CSB-EP455021DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP455022DUF
CSB-EP455022DUF
CSB-BP455022DUF
CSB-MP455022DUF
CSB-EP455022DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Carbon storage regulator homolog (csrA) CSB-YP455023DUF
CSB-EP455023DUF
CSB-BP455023DUF
CSB-MP455023DUF
CSB-EP455023DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Chaperone protein DnaJ (dnaJ) CSB-YP455024DUF
CSB-EP455024DUF
CSB-BP455024DUF
CSB-MP455024DUF
CSB-EP455024DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Potassium-transporting ATPase B chain (kdpB), partial CSB-YP455025DUF1
CSB-EP455025DUF1
CSB-BP455025DUF1
CSB-MP455025DUF1
CSB-EP455025DUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP455026DUF
CSB-EP455026DUF
CSB-BP455026DUF
CSB-MP455026DUF
CSB-EP455026DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribonuclease Z (rnz) CSB-YP455027DUF
CSB-EP455027DUF
CSB-BP455027DUF
CSB-MP455027DUF
CSB-EP455027DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP455028DUF
CSB-EP455028DUF
CSB-BP455028DUF
CSB-MP455028DUF
CSB-EP455028DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTPase Der (der) CSB-YP455029DUF
CSB-EP455029DUF
CSB-BP455029DUF
CSB-MP455029DUF
CSB-EP455029DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0296 protein CLH_1161 (CLH_1161) CSB-YP455030DUF
CSB-EP455030DUF
CSB-BP455030DUF
CSB-MP455030DUF
CSB-EP455030DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L19 (rplS) CSB-YP455031DUF
CSB-EP455031DUF
CSB-BP455031DUF
CSB-MP455031DUF
CSB-EP455031DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP455032DUF
CSB-EP455032DUF
CSB-BP455032DUF
CSB-MP455032DUF
CSB-EP455032DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Arginine repressor (argR) CSB-YP455033DUF
CSB-EP455033DUF
CSB-BP455033DUF
CSB-MP455033DUF
CSB-EP455033DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Bifunctional protein FolD (folD) CSB-YP455034DUF
CSB-EP455034DUF
CSB-BP455034DUF
CSB-MP455034DUF
CSB-EP455034DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum MutS2 protein (mutS2), partial CSB-YP455035DUF
CSB-EP455035DUF
CSB-BP455035DUF
CSB-MP455035DUF
CSB-EP455035DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Regulatory protein recX (recX) CSB-YP455036DUF
CSB-EP455036DUF
CSB-BP455036DUF
CSB-MP455036DUF
CSB-EP455036DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP455037FPP
CSB-EP455037FPP
CSB-BP455037FPP
CSB-MP455037FPP
CSB-EP455037FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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