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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Methylacidiphilum infernorum 50S ribosomal protein L6 (rplF) CSB-YP455218MSV
CSB-EP455218MSV
CSB-BP455218MSV
CSB-MP455218MSV
CSB-EP455218MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP455219MSV
CSB-EP455219MSV
CSB-BP455219MSV
CSB-MP455219MSV
CSB-EP455219MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Recombination protein RecR (recR) CSB-YP455220GFO
CSB-EP455220GFO
CSB-BP455220GFO
CSB-MP455220GFO
CSB-EP455220GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L20 (rplT) CSB-YP455221GFO
CSB-EP455221GFO
CSB-BP455221GFO
CSB-MP455221GFO
CSB-EP455221GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Homoserine O-acetyltransferase (metX) CSB-YP455222GFO
CSB-EP455222GFO
CSB-BP455222GFO
CSB-MP455222GFO
CSB-EP455222GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi UvrABC system protein C (uvrC), partial CSB-YP455223GFO
CSB-EP455223GFO
CSB-BP455223GFO
CSB-MP455223GFO
CSB-EP455223GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP455224GFO
CSB-EP455224GFO
CSB-BP455224GFO
CSB-MP455224GFO
CSB-EP455224GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi UPF0178 protein Glov_0658 (Glov_0658) CSB-YP455225GFO
CSB-EP455225GFO
CSB-BP455225GFO
CSB-MP455225GFO
CSB-EP455225GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Bifunctional protein GlmU (glmU) CSB-YP455226GFO
CSB-EP455226GFO
CSB-BP455226GFO
CSB-MP455226GFO
CSB-EP455226GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L28 (rpmB) CSB-YP455227GFO
CSB-EP455227GFO
CSB-BP455227GFO
CSB-MP455227GFO
CSB-EP455227GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Biotin synthase (bioB) CSB-YP455228GFO
CSB-EP455228GFO
CSB-BP455228GFO
CSB-MP455228GFO
CSB-EP455228GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Phosphoheptose isomerase (gmhA) CSB-YP455229GFO
CSB-EP455229GFO
CSB-BP455229GFO
CSB-MP455229GFO
CSB-EP455229GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Dihydroxy-acid dehydratase (ilvD), partial CSB-YP455230GFO
CSB-EP455230GFO
CSB-BP455230GFO
CSB-MP455230GFO
CSB-EP455230GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L25 (rplY) CSB-YP455231GFO
CSB-EP455231GFO
CSB-BP455231GFO
CSB-MP455231GFO
CSB-EP455231GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP455232GFO
CSB-EP455232GFO
CSB-BP455232GFO
CSB-MP455232GFO
CSB-EP455232GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Glycerol kinase (glpK) CSB-YP455233GFO
CSB-EP455233GFO
CSB-BP455233GFO
CSB-MP455233GFO
CSB-EP455233GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP455234GFO
CSB-EP455234GFO
CSB-BP455234GFO
CSB-MP455234GFO
CSB-EP455234GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Lon protease (lon), partial CSB-YP455235GFO
CSB-EP455235GFO
CSB-BP455235GFO
CSB-MP455235GFO
CSB-EP455235GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 30S ribosomal protein S7 (rpsG) CSB-YP455236GFO
CSB-EP455236GFO
CSB-BP455236GFO
CSB-MP455236GFO
CSB-EP455236GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L5 (rplE) CSB-YP455237GFO
CSB-EP455237GFO
CSB-BP455237GFO
CSB-MP455237GFO
CSB-EP455237GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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