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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila simulans Succinate dehydrogenase assembly factor 2-A, mitochondrial (GD10178) CSB-YP455799DMJ
CSB-EP455799DMJ
CSB-BP455799DMJ
CSB-MP455799DMJ
CSB-EP455799DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Calcium channel flower (flower), partial CSB-YP455800DMJ1
CSB-EP455800DMJ1
CSB-BP455800DMJ1
CSB-MP455800DMJ1
CSB-EP455800DMJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans SAGA-associated factor 11 homolog (Sgf11) CSB-YP455801DMJ
CSB-BP455801DMJ
CSB-MP455801DMJ
CSB-EP455801DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Eukaryotic translation initiation factor 3 subunit D-2 (eIF3-S7-2), partial CSB-YP455802DMJ
CSB-EP455802DMJ
CSB-BP455802DMJ
CSB-MP455802DMJ
CSB-EP455802DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Transcription factor kayak (kay) CSB-YP455803DMJ
CSB-EP455803DMJ
CSB-BP455803DMJ
CSB-MP455803DMJ
CSB-EP455803DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein Turandot Z (TotZ) CSB-YP455804DMJ
CSB-EP455804DMJ
CSB-BP455804DMJ
CSB-MP455804DMJ
CSB-EP455804DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans tRNA (guanine-N (7)-)-methyltransferase CSB-YP455805DMJ
CSB-EP455805DMJ
CSB-BP455805DMJ
CSB-MP455805DMJ
CSB-EP455805DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans DM7 family protein GD16138 (GD16138), partial CSB-YP455806DMJ
CSB-EP455806DMJ
CSB-BP455806DMJ
CSB-MP455806DMJ
CSB-EP455806DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 50S ribosomal protein L7/L12 (rplL) CSB-YP455807EUP
CSB-EP455807EUP
CSB-BP455807EUP
CSB-MP455807EUP
CSB-EP455807EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 50S ribosomal protein L4 (rplD) CSB-YP455808EUP
CSB-EP455808EUP
CSB-BP455808EUP
CSB-MP455808EUP
CSB-EP455808EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 50S ribosomal protein L16 (rplP) CSB-YP455809EUP
CSB-EP455809EUP
CSB-BP455809EUP
CSB-MP455809EUP
CSB-EP455809EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 50S ribosomal protein L6 (rplF) CSB-YP455810EUP
CSB-EP455810EUP
CSB-BP455810EUP
CSB-MP455810EUP
CSB-EP455810EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP455811EUP
CSB-EP455811EUP
CSB-BP455811EUP
CSB-MP455811EUP
CSB-EP455811EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP455812EUP
CSB-EP455812EUP
CSB-BP455812EUP
CSB-MP455812EUP
CSB-EP455812EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Phosphate acyltransferase (plsX) CSB-YP455813EUP
CSB-EP455813EUP
CSB-BP455813EUP
CSB-MP455813EUP
CSB-EP455813EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum D-amino acid dehydrogenase small subunit (dadA) CSB-YP455814EUP
CSB-EP455814EUP
CSB-BP455814EUP
CSB-MP455814EUP
CSB-EP455814EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Enolase (eno) CSB-YP455815EUP
CSB-EP455815EUP
CSB-BP455815EUP
CSB-MP455815EUP
CSB-EP455815EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Glutamate--tRNA ligase (gltX) CSB-YP455816EUP
CSB-EP455816EUP
CSB-BP455816EUP
CSB-MP455816EUP
CSB-EP455816EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Large-conductance mechanosensitive channel (mscL), partial CSB-YP455817EUP1
CSB-EP455817EUP1
CSB-BP455817EUP1
CSB-MP455817EUP1
CSB-EP455817EUP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP455818EUP
CSB-EP455818EUP
CSB-BP455818EUP
CSB-MP455818EUP
CSB-EP455818EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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