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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neisseria gonorrhoeae 30S ribosomal protein S21 (rpsU) CSB-YP455859NGB
CSB-EP455859NGB
CSB-BP455859NGB
CSB-MP455859NGB
CSB-EP455859NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Threonine--tRNA ligase (thrS), partial CSB-YP455860AZX
CSB-EP455860AZX
CSB-BP455860AZX
CSB-MP455860AZX
CSB-EP455860AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L5 (rplE) CSB-YP455861AZX
CSB-EP455861AZX
CSB-BP455861AZX
CSB-MP455861AZX
CSB-EP455861AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP455862AZX
CSB-EP455862AZX
CSB-BP455862AZX
CSB-MP455862AZX
CSB-EP455862AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Peptide chain release factor 3 (prfC), partial CSB-YP455863AZX
CSB-EP455863AZX
CSB-BP455863AZX
CSB-MP455863AZX
CSB-EP455863AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Bifunctional protein FolD (folD1) CSB-YP455864AZX
CSB-EP455864AZX
CSB-BP455864AZX
CSB-MP455864AZX
CSB-EP455864AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Protein syd (syd) CSB-YP455865AZX
CSB-EP455865AZX
CSB-BP455865AZX
CSB-MP455865AZX
CSB-EP455865AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii CCA-adding enzyme (cca) CSB-YP455866AZX
CSB-EP455866AZX
CSB-BP455866AZX
CSB-MP455866AZX
CSB-EP455866AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii S-adenosylmethionine synthase (metK) CSB-YP455867AZX
CSB-EP455867AZX
CSB-BP455867AZX
CSB-MP455867AZX
CSB-EP455867AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP455868AZX
CSB-EP455868AZX
CSB-BP455868AZX
CSB-MP455868AZX
CSB-EP455868AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L13 (rplM) CSB-YP455869AZX
CSB-EP455869AZX
CSB-BP455869AZX
CSB-MP455869AZX
CSB-EP455869AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii ATP-dependent protease ATPase subunit HslU (hslU1) CSB-YP455870AZX
CSB-EP455870AZX
CSB-BP455870AZX
CSB-MP455870AZX
CSB-EP455870AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP455871AZX
CSB-EP455871AZX
CSB-BP455871AZX
CSB-MP455871AZX
CSB-EP455871AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP455872ENT
CSB-EP455872ENT
CSB-BP455872ENT
CSB-MP455872ENT
CSB-EP455872ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Recombination protein RecR (recR) CSB-YP455873ENT
CSB-EP455873ENT
CSB-BP455873ENT
CSB-MP455873ENT
CSB-EP455873ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ureidoglycolate hydrolase (allA) CSB-YP455874ENT
CSB-EP455874ENT
CSB-BP455874ENT
CSB-MP455874ENT
CSB-EP455874ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multidrug resistance protein MdtK (mdtK), partial CSB-YP455875ENT
CSB-EP455875ENT
CSB-BP455875ENT
CSB-MP455875ENT
CSB-EP455875ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Integration host factor subunit alpha (ihfA) CSB-YP455876ENT
CSB-EP455876ENT
CSB-BP455876ENT
CSB-MP455876ENT
CSB-EP455876ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP455877ENT
CSB-EP455877ENT
CSB-BP455877ENT
CSB-MP455877ENT
CSB-EP455877ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP455878ENT
CSB-EP455878ENT
CSB-BP455878ENT
CSB-MP455878ENT
CSB-EP455878ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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