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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli NH (3)-dependent NAD (+) synthetase CSB-YP455879ENT
CSB-EP455879ENT
CSB-BP455879ENT
CSB-MP455879ENT
CSB-EP455879ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP455880ENT
CSB-EP455880ENT
CSB-BP455880ENT
CSB-MP455880ENT
CSB-EP455880ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0102 protein yraN (yraN) CSB-YP455881ENT
CSB-EP455881ENT
CSB-BP455881ENT
CSB-MP455881ENT
CSB-EP455881ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome-binding factor A (rbfA) CSB-YP455882ENT
CSB-EP455882ENT
CSB-BP455882ENT
CSB-MP455882ENT
CSB-EP455882ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0266 membrane protein yobD (yobD), partial CSB-YP455883ENT1
CSB-EP455883ENT1
CSB-BP455883ENT1
CSB-MP455883ENT1
CSB-EP455883ENT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP455884ENT
CSB-EP455884ENT
CSB-BP455884ENT
CSB-MP455884ENT
CSB-EP455884ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar transcriptional regulator FlhD (flhD) CSB-YP455885ENT
CSB-EP455885ENT
CSB-BP455885ENT
CSB-MP455885ENT
CSB-EP455885ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP455886ENT
CSB-EP455886ENT
CSB-BP455886ENT
CSB-MP455886ENT
CSB-EP455886ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP455887ENT1
CSB-EP455887ENT1
CSB-BP455887ENT1
CSB-MP455887ENT1
CSB-EP455887ENT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase gamma chain (atpG) CSB-YP455888FQA
CSB-EP455888FQA
CSB-BP455888FQA
CSB-MP455888FQA
CSB-EP455888FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosome maturation factor RimP (rimP) CSB-YP455889FQA
CSB-EP455889FQA
CSB-BP455889FQA
CSB-MP455889FQA
CSB-EP455889FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S4 (rpsD) CSB-YP455890FQA
CSB-EP455890FQA
CSB-BP455890FQA
CSB-MP455890FQA
CSB-EP455890FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L20 (rplT) CSB-YP455891FQA
CSB-EP455891FQA
CSB-BP455891FQA
CSB-MP455891FQA
CSB-EP455891FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center protein T (psbT), partial CSB-YP455892FQA
CSB-EP455892FQA
CSB-BP455892FQA
CSB-MP455892FQA
CSB-EP455892FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem I reaction center subunit IX (psaJ), partial CSB-YP455893FQA
CSB-EP455893FQA
CSB-BP455893FQA
CSB-MP455893FQA
CSB-EP455893FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L19 (rplS) CSB-YP455894FQA
CSB-EP455894FQA
CSB-BP455894FQA
CSB-MP455894FQA
CSB-EP455894FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L15 (rplO) CSB-YP455895FQA
CSB-EP455895FQA
CSB-BP455895FQA
CSB-MP455895FQA
CSB-EP455895FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S19 (rpsS) CSB-YP455896FQA
CSB-EP455896FQA
CSB-BP455896FQA
CSB-MP455896FQA
CSB-EP455896FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP455897FQA
CSB-EP455897FQA
CSB-BP455897FQA
CSB-MP455897FQA
CSB-EP455897FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 10 kDa chaperonin (groS) CSB-YP455898FQA
CSB-EP455898FQA
CSB-BP455898FQA
CSB-MP455898FQA
CSB-EP455898FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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