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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 Primosomal replication protein n (priB) CSB-YP457220AXI
CSB-EP457220AXI
CSB-BP457220AXI
CSB-MP457220AXI
CSB-EP457220AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP457221AXI
CSB-EP457221AXI
CSB-BP457221AXI
CSB-MP457221AXI
CSB-EP457221AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Valine--tRNA ligase (valS), partial CSB-YP457222AXI
CSB-EP457222AXI
CSB-BP457222AXI
CSB-MP457222AXI
CSB-EP457222AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Pantothenate kinase (coaA) CSB-YP457223AXI
CSB-EP457223AXI
CSB-BP457223AXI
CSB-MP457223AXI
CSB-EP457223AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal protein L11 methyltransferase (prmA) CSB-YP457224AXI
CSB-EP457224AXI
CSB-BP457224AXI
CSB-MP457224AXI
CSB-EP457224AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 L-seryl-tRNA (Sec) selenium transferase CSB-YP457225AXI
CSB-EP457225AXI
CSB-BP457225AXI
CSB-MP457225AXI
CSB-EP457225AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ATP-dependent protease subunit HslV (hslV) CSB-YP457226AXI
CSB-EP457226AXI
CSB-BP457226AXI
CSB-MP457226AXI
CSB-EP457226AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L22 (rplV) CSB-YP457227AXI
CSB-EP457227AXI
CSB-BP457227AXI
CSB-MP457227AXI
CSB-EP457227AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L24 (rplX) CSB-YP457228AXI
CSB-EP457228AXI
CSB-BP457228AXI
CSB-MP457228AXI
CSB-EP457228AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L17 (rplQ) CSB-YP457229AXI
CSB-EP457229AXI
CSB-BP457229AXI
CSB-MP457229AXI
CSB-EP457229AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP457230AXI
CSB-EP457230AXI
CSB-BP457230AXI
CSB-MP457230AXI
CSB-EP457230AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Proline--tRNA ligase (proS), partial CSB-YP457231AXI
CSB-EP457231AXI
CSB-BP457231AXI
CSB-MP457231AXI
CSB-EP457231AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glutamate racemase (murI) CSB-YP457232AXI
CSB-EP457232AXI
CSB-BP457232AXI
CSB-MP457232AXI
CSB-EP457232AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Sec-independent protein translocase protein TatA (tatA), partial CSB-YP457233AXI
CSB-EP457233AXI
CSB-BP457233AXI
CSB-MP457233AXI
CSB-EP457233AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP457234AXI
CSB-EP457234AXI
CSB-BP457234AXI
CSB-MP457234AXI
CSB-EP457234AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP457235AXI
CSB-EP457235AXI
CSB-BP457235AXI
CSB-MP457235AXI
CSB-EP457235AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Dihydroxy-acid dehydratase (ilvD), partial CSB-YP457236AXI
CSB-EP457236AXI
CSB-BP457236AXI
CSB-MP457236AXI
CSB-EP457236AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP457237AXI
CSB-EP457237AXI
CSB-BP457237AXI
CSB-MP457237AXI
CSB-EP457237AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 N utilization substance protein B homolog (nusB) CSB-YP457238AXI
CSB-EP457238AXI
CSB-BP457238AXI
CSB-MP457238AXI
CSB-EP457238AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Threonine--tRNA ligase (thrS), partial CSB-YP457239AXI
CSB-EP457239AXI
CSB-BP457239AXI
CSB-MP457239AXI
CSB-EP457239AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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