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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phenylobacterium zucineum NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP457742EUP1
CSB-EP457742EUP1
CSB-BP457742EUP1
CSB-MP457742EUP1
CSB-EP457742EUP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum DNA repair protein recO (recO) CSB-YP457743EUP
CSB-EP457743EUP
CSB-BP457743EUP
CSB-MP457743EUP
CSB-EP457743EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Sec-independent protein translocase protein TatB (tatB), partial CSB-YP457744EUP
CSB-EP457744EUP
CSB-BP457744EUP
CSB-MP457744EUP
CSB-EP457744EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum DNA ligase (ligA), partial CSB-YP457745EUP
CSB-EP457745EUP
CSB-BP457745EUP
CSB-MP457745EUP
CSB-EP457745EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP457746EUP
CSB-EP457746EUP
CSB-BP457746EUP
CSB-MP457746EUP
CSB-EP457746EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP457747EUP
CSB-EP457747EUP
CSB-BP457747EUP
CSB-MP457747EUP
CSB-EP457747EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Elongation factor P (efp) CSB-YP457748EUP
CSB-EP457748EUP
CSB-BP457748EUP
CSB-MP457748EUP
CSB-EP457748EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae tRNA (guanine-N (1)-)-methyltransferase CSB-YP457749NGB
CSB-EP457749NGB
CSB-BP457749NGB
CSB-MP457749NGB
CSB-EP457749NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP457750NGB
CSB-EP457750NGB
CSB-BP457750NGB
CSB-MP457750NGB
CSB-EP457750NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Peptide chain release factor 3 (prfC), partial CSB-YP457751NGB
CSB-EP457751NGB
CSB-BP457751NGB
CSB-MP457751NGB
CSB-EP457751NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP457752NGB
CSB-EP457752NGB
CSB-BP457752NGB
CSB-MP457752NGB
CSB-EP457752NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP457753NGB
CSB-EP457753NGB
CSB-BP457753NGB
CSB-MP457753NGB
CSB-EP457753NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP457754NGB
CSB-EP457754NGB
CSB-BP457754NGB
CSB-MP457754NGB
CSB-EP457754NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP457755NGB
CSB-EP457755NGB
CSB-BP457755NGB
CSB-MP457755NGB
CSB-EP457755NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP457756NGB
CSB-EP457756NGB
CSB-BP457756NGB
CSB-MP457756NGB
CSB-EP457756NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Protein translocase subunit SecA (secA), partial CSB-YP457757NGB
CSB-EP457757NGB
CSB-BP457757NGB
CSB-MP457757NGB
CSB-EP457757NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP457758NGB
CSB-EP457758NGB
CSB-BP457758NGB
CSB-MP457758NGB
CSB-EP457758NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP457759NGB1
CSB-EP457759NGB1
CSB-BP457759NGB1
CSB-MP457759NGB1
CSB-EP457759NGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Recombination protein RecR (recR) CSB-YP457760NGB
CSB-EP457760NGB
CSB-BP457760NGB
CSB-MP457760NGB
CSB-EP457760NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 3-dehydroquinate dehydratase (aroD) CSB-YP457761NGB
CSB-EP457761NGB
CSB-BP457761NGB
CSB-MP457761NGB
CSB-EP457761NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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