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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila persimilis Succinate dehydrogenase assembly factor 2-A, mitochondrial (GL10881) CSB-YP457641DMB
CSB-EP457641DMB
CSB-BP457641DMB
CSB-MP457641DMB
CSB-EP457641DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Succinate dehydrogenase assembly factor 2-B, mitochondrial (GL17204) CSB-YP457642DMB
CSB-EP457642DMB
CSB-BP457642DMB
CSB-MP457642DMB
CSB-EP457642DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta (PrBP) CSB-YP457643DMB
CSB-EP457643DMB
CSB-BP457643DMB
CSB-MP457643DMB
CSB-EP457643DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Kelch-like protein diablo (dbo), partial CSB-YP457644DMB
CSB-EP457644DMB
CSB-BP457644DMB
CSB-MP457644DMB
CSB-EP457644DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis NAD (P)H-hydrate epimerase CSB-YP457645DMB
CSB-EP457645DMB
CSB-BP457645DMB
CSB-MP457645DMB
CSB-EP457645DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Probable cytosolic Fe-S cluster assembly factor GL21135 (GL21135) CSB-YP457646DMB
CSB-EP457646DMB
CSB-BP457646DMB
CSB-MP457646DMB
CSB-EP457646DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Histone-arginine methyltransferase CARMER (Art4), partial CSB-YP457647DMB
CSB-EP457647DMB
CSB-BP457647DMB
CSB-MP457647DMB
CSB-EP457647DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial (GL15483), partial CSB-YP457648DMB
CSB-EP457648DMB
CSB-BP457648DMB
CSB-MP457648DMB
CSB-EP457648DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Histone-arginine methyltransferase CARMER (Art4), partial CSB-YP457649DMG
CSB-EP457649DMG
CSB-BP457649DMG
CSB-MP457649DMG
CSB-EP457649DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Queuine tRNA-ribosyltransferase subunit QTRTD1 homolog (GM24868) CSB-YP457650DMG
CSB-EP457650DMG
CSB-BP457650DMG
CSB-MP457650DMG
CSB-EP457650DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Ribosome biogenesis protein BOP1 homolog (GM21825), partial CSB-YP457651DMG
CSB-EP457651DMG
CSB-BP457651DMG
CSB-MP457651DMG
CSB-EP457651DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein CLP1 homolog (cbc) CSB-YP457652DMG
CSB-EP457652DMG
CSB-BP457652DMG
CSB-MP457652DMG
CSB-EP457652DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Putative N (4)- (beta-N-acetylglucosaminyl)-L-asparaginase GM21137 CSB-YP457653DMG
CSB-EP457653DMG
CSB-BP457653DMG
CSB-MP457653DMG
CSB-EP457653DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Anamorsin homolog (l (2)35Bg) CSB-YP457654DMG
CSB-EP457654DMG
CSB-BP457654DMG
CSB-MP457654DMG
CSB-EP457654DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta (PrBP) CSB-YP457655DMG
CSB-EP457655DMG
CSB-BP457655DMG
CSB-MP457655DMG
CSB-EP457655DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein hook (hk), partial CSB-YP457656DMG
CSB-EP457656DMG
CSB-BP457656DMG
CSB-MP457656DMG
CSB-EP457656DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia L-asparaginase-like protein GM15681 (GM15681) CSB-YP457657DMG
CSB-EP457657DMG
CSB-BP457657DMG
CSB-MP457657DMG
CSB-EP457657DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Lipoyl synthase, mitochondrial (Las), partial CSB-YP457658DMG
CSB-EP457658DMG
CSB-BP457658DMG
CSB-MP457658DMG
CSB-EP457658DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Molybdopterin synthase sulfur carrier subunit (Mocs2) CSB-YP457659DMG
CSB-EP457659DMG
CSB-BP457659DMG
CSB-MP457659DMG
CSB-EP457659DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Neuropathy target esterase sws (sws), partial CSB-YP457660DMG
CSB-EP457660DMG
CSB-BP457660DMG
CSB-MP457660DMG
CSB-EP457660DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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