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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylobacterium populi Heme A synthase (ctaA), partial CSB-YP457981MTC
CSB-EP457981MTC
CSB-BP457981MTC
CSB-MP457981MTC
CSB-EP457981MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP457982MTC
CSB-EP457982MTC
CSB-BP457982MTC
CSB-MP457982MTC
CSB-EP457982MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP457983MTC
CSB-EP457983MTC
CSB-BP457983MTC
CSB-MP457983MTC
CSB-EP457983MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP457984MTC
CSB-EP457984MTC
CSB-BP457984MTC
CSB-MP457984MTC
CSB-EP457984MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Serine--tRNA ligase (serS) CSB-YP457985MTC
CSB-EP457985MTC
CSB-BP457985MTC
CSB-MP457985MTC
CSB-EP457985MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP457986MTC
CSB-EP457986MTC
CSB-BP457986MTC
CSB-MP457986MTC
CSB-EP457986MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP457987MTC
CSB-EP457987MTC
CSB-BP457987MTC
CSB-MP457987MTC
CSB-EP457987MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi FMN-dependent NADH-azoreductase (azoR) CSB-YP457988MTC
CSB-EP457988MTC
CSB-BP457988MTC
CSB-MP457988MTC
CSB-EP457988MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP457989MTC
CSB-EP457989MTC
CSB-BP457989MTC
CSB-MP457989MTC
CSB-EP457989MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi UPF0235 protein Mpop_2087 (Mpop_2087) CSB-YP457990MTC
CSB-EP457990MTC
CSB-BP457990MTC
CSB-MP457990MTC
CSB-EP457990MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae 10 kDa chaperonin (groS) CSB-YP457991OEN
CSB-EP457991OEN
CSB-BP457991OEN
CSB-MP457991OEN
CSB-EP457991OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP457992OEN
CSB-EP457992OEN
CSB-BP457992OEN
CSB-MP457992OEN
CSB-EP457992OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae 30S ribosomal protein S8 (rpsH) CSB-YP457993OEN
CSB-EP457993OEN
CSB-BP457993OEN
CSB-MP457993OEN
CSB-EP457993OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae 50S ribosomal protein L22 (rplV) CSB-YP457994OEN
CSB-EP457994OEN
CSB-BP457994OEN
CSB-MP457994OEN
CSB-EP457994OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae UPF0173 metal-dependent hydrolase Oter_4201 (Oter_4201) CSB-YP457995OEN
CSB-EP457995OEN
CSB-BP457995OEN
CSB-MP457995OEN
CSB-EP457995OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP457996OEN
CSB-EP457996OEN
CSB-BP457996OEN
CSB-MP457996OEN
CSB-EP457996OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP457997OEN
CSB-EP457997OEN
CSB-BP457997OEN
CSB-MP457997OEN
CSB-EP457997OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP457998OEN
CSB-EP457998OEN
CSB-BP457998OEN
CSB-MP457998OEN
CSB-EP457998OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP457999OEN
CSB-EP457999OEN
CSB-BP457999OEN
CSB-MP457999OEN
CSB-EP457999OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Ribosomal protein L11 methyltransferase (prmA) CSB-YP458000OEN
CSB-EP458000OEN
CSB-BP458000OEN
CSB-MP458000OEN
CSB-EP458000OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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