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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia phymatum Diaminopimelate epimerase (dapF) CSB-YP460341BXT
CSB-EP460341BXT
CSB-BP460341BXT
CSB-MP460341BXT
CSB-EP460341BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 8-amino-7-oxononanoate synthase (bioF) CSB-YP460342BXT
CSB-EP460342BXT
CSB-BP460342BXT
CSB-MP460342BXT
CSB-EP460342BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ribose-5-phosphate isomerase A (rpiA) CSB-YP460343BXT
CSB-EP460343BXT
CSB-BP460343BXT
CSB-MP460343BXT
CSB-EP460343BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum PKHD-type hydroxylase Bphy_5374 (Bphy_5374) CSB-YP460344BXT
CSB-EP460344BXT
CSB-BP460344BXT
CSB-MP460344BXT
CSB-EP460344BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP460345BXT
CSB-EP460345BXT
CSB-BP460345BXT
CSB-MP460345BXT
CSB-EP460345BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Acetaldehyde dehydrogenase 2 (Bphy_4538) CSB-YP460346BXT
CSB-EP460346BXT
CSB-BP460346BXT
CSB-MP460346BXT
CSB-EP460346BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Putative 3-methyladenine DNA glycosylase (Bphy_3243) CSB-YP460347BXT
CSB-EP460347BXT
CSB-BP460347BXT
CSB-MP460347BXT
CSB-EP460347BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Der GTPase-activating protein YihI (yihI) CSB-YP460348YAW
CSB-EP460348YAW
CSB-BP460348YAW
CSB-MP460348YAW
CSB-EP460348YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Orotate phosphoribosyltransferase (pyrE) CSB-YP460349YAW
CSB-EP460349YAW
CSB-BP460349YAW
CSB-MP460349YAW
CSB-EP460349YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP460350YAW
CSB-EP460350YAW
CSB-BP460350YAW
CSB-MP460350YAW
CSB-EP460350YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Methylglyoxal synthase (mgsA) CSB-YP460351YAW
CSB-EP460351YAW
CSB-BP460351YAW
CSB-MP460351YAW
CSB-EP460351YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ribonuclease HII (rnhB) CSB-YP460352YAW
CSB-EP460352YAW
CSB-BP460352YAW
CSB-MP460352YAW
CSB-EP460352YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 30S ribosomal protein S2 (rpsB) CSB-YP460353YAW
CSB-EP460353YAW
CSB-BP460353YAW
CSB-MP460353YAW
CSB-EP460353YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Amino-acid acetyltransferase (argA) CSB-YP460354YAW
CSB-EP460354YAW
CSB-BP460354YAW
CSB-MP460354YAW
CSB-EP460354YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Lysophospholipid transporter lplT (lplT), partial CSB-YP460355YAW
CSB-EP460355YAW
CSB-BP460355YAW
CSB-MP460355YAW
CSB-EP460355YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Regulator of ribonuclease activity A (rraA) CSB-YP460356YAW
CSB-EP460356YAW
CSB-BP460356YAW
CSB-MP460356YAW
CSB-EP460356YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Deoxycytidine triphosphate deaminase (dcd) CSB-YP460357YAW
CSB-EP460357YAW
CSB-BP460357YAW
CSB-MP460357YAW
CSB-EP460357YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Lipid A palmitoyltransferase PagP (pagP) CSB-YP460358YAW
CSB-EP460358YAW
CSB-BP460358YAW
CSB-MP460358YAW
CSB-EP460358YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB S-adenosylmethionine synthase (metK) CSB-YP460359YAW
CSB-EP460359YAW
CSB-BP460359YAW
CSB-MP460359YAW
CSB-EP460359YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP460360YAW
CSB-EP460360YAW
CSB-BP460360YAW
CSB-MP460360YAW
CSB-EP460360YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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