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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Francisella tularensis subsp. mediasiatica Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP458602FFF
CSB-EP458602FFF
CSB-BP458602FFF
CSB-MP458602FFF
CSB-EP458602FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Outer-membrane lipoprotein carrier protein (lolA) CSB-YP458603FFF
CSB-EP458603FFF
CSB-BP458603FFF
CSB-MP458603FFF
CSB-EP458603FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 50S ribosomal protein L33 (rpmG) CSB-YP458604FFF
CSB-EP458604FFF
CSB-BP458604FFF
CSB-MP458604FFF
CSB-EP458604FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP458605FFF
CSB-EP458605FFF
CSB-BP458605FFF
CSB-MP458605FFF
CSB-EP458605FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 50S ribosomal protein L32 (rpmF) CSB-YP458606FFF
CSB-EP458606FFF
CSB-BP458606FFF
CSB-MP458606FFF
CSB-EP458606FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Lipid-A-disaccharide synthase (lpxB) CSB-YP458607FFF
CSB-EP458607FFF
CSB-BP458607FFF
CSB-MP458607FFF
CSB-EP458607FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica UPF0434 protein FTM_0733 (FTM_0733) CSB-YP458608FFF
CSB-EP458608FFF
CSB-BP458608FFF
CSB-MP458608FFF
CSB-EP458608FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP458609FFF
CSB-EP458609FFF
CSB-BP458609FFF
CSB-MP458609FFF
CSB-EP458609FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP458610FFF
CSB-EP458610FFF
CSB-BP458610FFF
CSB-MP458610FFF
CSB-EP458610FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP458611FFF
CSB-EP458611FFF
CSB-BP458611FFF
CSB-MP458611FFF
CSB-EP458611FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 50S ribosomal protein L35 (rpmI) CSB-YP458612FFF
CSB-EP458612FFF
CSB-BP458612FFF
CSB-MP458612FFF
CSB-EP458612FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Protein grpE (grpE) CSB-YP458613FFF
CSB-EP458613FFF
CSB-BP458613FFF
CSB-MP458613FFF
CSB-EP458613FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica DNA polymerase IV (dinB) CSB-YP458614FFF
CSB-EP458614FFF
CSB-BP458614FFF
CSB-MP458614FFF
CSB-EP458614FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Probable transcriptional regulatory protein FTM_1203 (FTM_1203) CSB-YP458615FFF
CSB-EP458615FFF
CSB-BP458615FFF
CSB-MP458615FFF
CSB-EP458615FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP458616XBC
CSB-EP458616XBC
CSB-BP458616XBC
CSB-MP458616XBC
CSB-EP458616XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP458617XBC
CSB-EP458617XBC
CSB-BP458617XBC
CSB-MP458617XBC
CSB-EP458617XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Sec-independent protein translocase protein TatA (tatA), partial CSB-YP458618XBC
CSB-EP458618XBC
CSB-BP458618XBC
CSB-MP458618XBC
CSB-EP458618XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 60 kDa chaperonin (groL), partial CSB-YP458619XBC
CSB-EP458619XBC
CSB-BP458619XBC
CSB-MP458619XBC
CSB-EP458619XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Shikimate kinase (aroK) CSB-YP458620XBC
CSB-EP458620XBC
CSB-BP458620XBC
CSB-MP458620XBC
CSB-EP458620XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP458621XBC
CSB-EP458621XBC
CSB-BP458621XBC
CSB-MP458621XBC
CSB-EP458621XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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