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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptothrix cholodnii Indole-3-glycerol phosphate synthase (trpC) CSB-YP461781LOT
CSB-EP461781LOT
CSB-BP461781LOT
CSB-MP461781LOT
CSB-EP461781LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP461782LOT
CSB-EP461782LOT
CSB-BP461782LOT
CSB-MP461782LOT
CSB-EP461782LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP461783LOT
CSB-EP461783LOT
CSB-BP461783LOT
CSB-MP461783LOT
CSB-EP461783LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S13 (rpsM) CSB-YP461784LOT
CSB-EP461784LOT
CSB-BP461784LOT
CSB-MP461784LOT
CSB-EP461784LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S8 (rpsH) CSB-YP461785LOT
CSB-EP461785LOT
CSB-BP461785LOT
CSB-MP461785LOT
CSB-EP461785LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP461786LOT
CSB-EP461786LOT
CSB-BP461786LOT
CSB-MP461786LOT
CSB-EP461786LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L3 (rplC) CSB-YP461787LOT
CSB-EP461787LOT
CSB-BP461787LOT
CSB-MP461787LOT
CSB-EP461787LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Probable deoxycytidine triphosphate deaminase (dcd) CSB-YP461788TNE
CSB-EP461788TNE
CSB-BP461788TNE
CSB-MP461788TNE
CSB-EP461788TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L37Ae (rpl37Ae) CSB-YP461789TNE
CSB-EP461789TNE
CSB-BP461789TNE
CSB-MP461789TNE
CSB-EP461789TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP461790TNE
CSB-EP461790TNE
CSB-BP461790TNE
CSB-MP461790TNE
CSB-EP461790TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S4e (rps4e) CSB-YP461791TNE
CSB-EP461791TNE
CSB-BP461791TNE
CSB-MP461791TNE
CSB-EP461791TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L7Ae (rpl7Ae) CSB-YP461792TNE
CSB-EP461792TNE
CSB-BP461792TNE
CSB-MP461792TNE
CSB-EP461792TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP461793TNE
CSB-EP461793TNE
CSB-BP461793TNE
CSB-MP461793TNE
CSB-EP461793TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L14e (rpl14e) CSB-YP461794TNE
CSB-EP461794TNE
CSB-BP461794TNE
CSB-MP461794TNE
CSB-EP461794TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus DNA-binding protein Tneu_1679 (Tneu_1679) CSB-YP461795TNE
CSB-EP461795TNE
CSB-BP461795TNE
CSB-MP461795TNE
CSB-EP461795TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP461796TNE
CSB-EP461796TNE
CSB-BP461796TNE
CSB-MP461796TNE
CSB-EP461796TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Deoxyribose-phosphate aldolase (deoC) CSB-YP461797TNE
CSB-EP461797TNE
CSB-BP461797TNE
CSB-MP461797TNE
CSB-EP461797TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Glutamyl-tRNA reductase (hemA) CSB-YP461798TNE
CSB-EP461798TNE
CSB-BP461798TNE
CSB-MP461798TNE
CSB-EP461798TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Elongation factor 1-beta (ef1b) CSB-YP461799TNE
CSB-EP461799TNE
CSB-BP461799TNE
CSB-MP461799TNE
CSB-EP461799TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S6e (rps6e) CSB-YP461800TNE
CSB-EP461800TNE
CSB-BP461800TNE
CSB-MP461800TNE
CSB-EP461800TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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