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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Proteus mirabilis ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP461521EYZ
CSB-EP461521EYZ
CSB-BP461521EYZ
CSB-MP461521EYZ
CSB-EP461521EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP461522EYZ
CSB-EP461522EYZ
CSB-BP461522EYZ
CSB-MP461522EYZ
CSB-EP461522EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP461523EYZ
CSB-EP461523EYZ
CSB-BP461523EYZ
CSB-MP461523EYZ
CSB-EP461523EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Acetylglutamate kinase (argB) CSB-YP461524EYZ
CSB-EP461524EYZ
CSB-BP461524EYZ
CSB-MP461524EYZ
CSB-EP461524EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L3 (rplC) CSB-YP461525EYZ
CSB-EP461525EYZ
CSB-BP461525EYZ
CSB-MP461525EYZ
CSB-EP461525EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 30S ribosomal protein S8 (rpsH) CSB-YP461526EYZ
CSB-EP461526EYZ
CSB-BP461526EYZ
CSB-MP461526EYZ
CSB-EP461526EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP461527EYZ
CSB-EP461527EYZ
CSB-BP461527EYZ
CSB-MP461527EYZ
CSB-EP461527EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis tRNA pseudouridine synthase D (truD) CSB-YP461528EYZ
CSB-EP461528EYZ
CSB-BP461528EYZ
CSB-MP461528EYZ
CSB-EP461528EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Primosomal replication protein n (priB) CSB-YP461529EYZ
CSB-EP461529EYZ
CSB-BP461529EYZ
CSB-MP461529EYZ
CSB-EP461529EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP461530EYZ
CSB-EP461530EYZ
CSB-BP461530EYZ
CSB-MP461530EYZ
CSB-EP461530EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis GTPase obg (obg) CSB-YP461531EYZ
CSB-EP461531EYZ
CSB-BP461531EYZ
CSB-MP461531EYZ
CSB-EP461531EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP461532EYZ
CSB-EP461532EYZ
CSB-BP461532EYZ
CSB-MP461532EYZ
CSB-EP461532EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP461533EYZ1
CSB-EP461533EYZ1
CSB-BP461533EYZ1
CSB-MP461533EYZ1
CSB-EP461533EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Regulator of ribonuclease activity B (rraB) CSB-YP461534EYZ
CSB-EP461534EYZ
CSB-BP461534EYZ
CSB-MP461534EYZ
CSB-EP461534EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP461535EYZ
CSB-EP461535EYZ
CSB-BP461535EYZ
CSB-MP461535EYZ
CSB-EP461535EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays CASP-like protein 4, partial CSB-YP461536ZAX1
CSB-EP461536ZAX1
CSB-BP461536ZAX1
CSB-MP461536ZAX1
CSB-EP461536ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1, partial CSB-YP461537ZAX
CSB-EP461537ZAX
CSB-BP461537ZAX
CSB-MP461537ZAX
CSB-EP461537ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Serine protease HTRA2, mitochondrial (HtrA2), partial CSB-YP461538DMB1
CSB-EP461538DMB1
CSB-BP461538DMB1
CSB-MP461538DMB1
CSB-EP461538DMB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Adenylosuccinate synthetase (GL24277) CSB-YP461539DMB
CSB-EP461539DMB
CSB-BP461539DMB
CSB-MP461539DMB
CSB-EP461539DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Protein KIAA0664 homolog (GL11405), partial CSB-YP461540DMB
CSB-EP461540DMB
CSB-BP461540DMB
CSB-MP461540DMB
CSB-EP461540DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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