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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xylella fastidiosa Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP462122XBN1
CSB-EP462122XBN1
CSB-BP462122XBN1
CSB-MP462122XBN1
CSB-EP462122XBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Type III pantothenate kinase (coaX) CSB-YP462123XBN
CSB-EP462123XBN
CSB-BP462123XBN
CSB-MP462123XBN
CSB-EP462123XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP462124XBN
CSB-EP462124XBN
CSB-BP462124XBN
CSB-MP462124XBN
CSB-EP462124XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP462125XBN
CSB-EP462125XBN
CSB-BP462125XBN
CSB-MP462125XBN
CSB-EP462125XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative Holliday junction resolvase (XfasM23_1360) CSB-YP462126XBN
CSB-EP462126XBN
CSB-BP462126XBN
CSB-MP462126XBN
CSB-EP462126XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP462127XBN
CSB-EP462127XBN
CSB-BP462127XBN
CSB-MP462127XBN
CSB-EP462127XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 3-dehydroquinate dehydratase (aroQ) CSB-YP462128XBN
CSB-EP462128XBN
CSB-BP462128XBN
CSB-MP462128XBN
CSB-EP462128XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein hfq (hfq) CSB-YP462129XBN
CSB-EP462129XBN
CSB-BP462129XBN
CSB-MP462129XBN
CSB-EP462129XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA (guanine-N (1)-)-methyltransferase CSB-YP462130XBN
CSB-EP462130XBN
CSB-BP462130XBN
CSB-MP462130XBN
CSB-EP462130XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Valine--tRNA ligase (valS), partial CSB-YP462131XBN
CSB-EP462131XBN
CSB-BP462131XBN
CSB-MP462131XBN
CSB-EP462131XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Peptide chain release factor 3 (prfC), partial CSB-YP462132XBN
CSB-EP462132XBN
CSB-BP462132XBN
CSB-MP462132XBN
CSB-EP462132XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa GTPase obg (obg) CSB-YP462133XBN
CSB-EP462133XBN
CSB-BP462133XBN
CSB-MP462133XBN
CSB-EP462133XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L36 (rpmJ) CSB-YP462134XBN
CSB-EP462134XBN
CSB-BP462134XBN
CSB-MP462134XBN
CSB-EP462134XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP462135XBN
CSB-EP462135XBN
CSB-BP462135XBN
CSB-MP462135XBN
CSB-EP462135XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP462136XBN
CSB-EP462136XBN
CSB-BP462136XBN
CSB-MP462136XBN
CSB-EP462136XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP462137XBN
CSB-EP462137XBN
CSB-BP462137XBN
CSB-MP462137XBN
CSB-EP462137XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP462138XBN
CSB-EP462138XBN
CSB-BP462138XBN
CSB-MP462138XBN
CSB-EP462138XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP synthase gamma chain (atpG) CSB-YP462139XBN
CSB-EP462139XBN
CSB-BP462139XBN
CSB-MP462139XBN
CSB-EP462139XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa RNA pyrophosphohydrolase (rppH) CSB-YP462140XBN
CSB-EP462140XBN
CSB-BP462140XBN
CSB-MP462140XBN
CSB-EP462140XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L23 (rplW) CSB-YP462141XBN
CSB-EP462141XBN
CSB-BP462141XBN
CSB-MP462141XBN
CSB-EP462141XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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