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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP462522XBC
CSB-EP462522XBC
CSB-BP462522XBC
CSB-MP462522XBC
CSB-EP462522XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP462523XBC
CSB-EP462523XBC
CSB-BP462523XBC
CSB-MP462523XBC
CSB-EP462523XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Regulatory protein recX (recX) CSB-YP462524XBC
CSB-EP462524XBC
CSB-BP462524XBC
CSB-MP462524XBC
CSB-EP462524XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae UvrABC system protein B (uvrB), partial CSB-YP462525XBC
CSB-EP462525XBC
CSB-BP462525XBC
CSB-MP462525XBC
CSB-EP462525XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP462526XBC
CSB-EP462526XBC
CSB-BP462526XBC
CSB-MP462526XBC
CSB-EP462526XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Lysine--tRNA ligase (lysS), partial CSB-YP462527XBC
CSB-EP462527XBC
CSB-BP462527XBC
CSB-MP462527XBC
CSB-EP462527XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP462528XBC
CSB-EP462528XBC
CSB-BP462528XBC
CSB-MP462528XBC
CSB-EP462528XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Tryptophan synthase alpha chain (trpA) CSB-YP462529XBC
CSB-EP462529XBC
CSB-BP462529XBC
CSB-MP462529XBC
CSB-EP462529XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Glycogen synthase (glgA), partial CSB-YP462530XBC
CSB-EP462530XBC
CSB-BP462530XBC
CSB-MP462530XBC
CSB-EP462530XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP462531XBC
CSB-EP462531XBC
CSB-BP462531XBC
CSB-MP462531XBC
CSB-EP462531XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 8-amino-7-oxononanoate synthase (bioF) CSB-YP462532BXU
CSB-EP462532BXU
CSB-BP462532BXU
CSB-MP462532BXU
CSB-EP462532BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Protein-export protein secB (secB) CSB-YP462533BXU
CSB-EP462533BXU
CSB-BP462533BXU
CSB-MP462533BXU
CSB-EP462533BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP462534BXU
CSB-EP462534BXU
CSB-BP462534BXU
CSB-MP462534BXU
CSB-EP462534BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP462535BXU1
CSB-EP462535BXU1
CSB-BP462535BXU1
CSB-MP462535BXU1
CSB-EP462535BXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP462536BXU
CSB-EP462536BXU
CSB-BP462536BXU
CSB-MP462536BXU
CSB-EP462536BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Proline--tRNA ligase (proS), partial CSB-YP462537BXU
CSB-EP462537BXU
CSB-BP462537BXU
CSB-MP462537BXU
CSB-EP462537BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP462538BXU
CSB-EP462538BXU
CSB-BP462538BXU
CSB-MP462538BXU
CSB-EP462538BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP462539BXU
CSB-EP462539BXU
CSB-BP462539BXU
CSB-MP462539BXU
CSB-EP462539BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP462540BXU
CSB-EP462540BXU
CSB-BP462540BXU
CSB-MP462540BXU
CSB-EP462540BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP462541BXU
CSB-EP462541BXU
CSB-BP462541BXU
CSB-MP462541BXU
CSB-EP462541BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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