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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila persimilis Latrophilin Cirl (Cirl), partial CSB-YP461541DMB
CSB-EP461541DMB
CSB-BP461541DMB
CSB-MP461541DMB
CSB-EP461541DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Transmembrane emp24 domain-containing protein bai (bai), partial CSB-YP461542DMB
CSB-EP461542DMB
CSB-BP461542DMB
CSB-MP461542DMB
CSB-EP461542DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Protein asunder (asun), partial CSB-YP461543DMB
CSB-EP461543DMB
CSB-BP461543DMB
CSB-MP461543DMB
CSB-EP461543DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 homolog (GL16887), partial CSB-YP461544DMB
CSB-EP461544DMB
CSB-BP461544DMB
CSB-MP461544DMB
CSB-EP461544DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis WD repeat-containing protein 48 homolog (GL16745), partial CSB-YP461545DMB
CSB-EP461545DMB
CSB-BP461545DMB
CSB-MP461545DMB
CSB-EP461545DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Interference hedgehog (iHog), partial CSB-YP461546DMB
CSB-EP461546DMB
CSB-BP461546DMB
CSB-MP461546DMB
CSB-EP461546DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis WD repeat-containing protein 55 homolog (GL12389), partial CSB-YP461547DMB
CSB-EP461547DMB
CSB-BP461547DMB
CSB-MP461547DMB
CSB-EP461547DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Calcium channel flower (flower), partial CSB-YP461548DMB1
CSB-EP461548DMB1
CSB-BP461548DMB1
CSB-MP461548DMB1
CSB-EP461548DMB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Protein cereblon homolog (GL27201), partial CSB-YP461549DMB
CSB-EP461549DMB
CSB-BP461549DMB
CSB-MP461549DMB
CSB-EP461549DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Queuine tRNA-ribosyltransferase subunit QTRTD1 homolog (GL17966) CSB-YP461550DMB
CSB-EP461550DMB
CSB-BP461550DMB
CSB-MP461550DMB
CSB-EP461550DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Serine protease HTRA2, mitochondrial (HtrA2), partial CSB-YP461551DMG1
CSB-EP461551DMG1
CSB-BP461551DMG1
CSB-MP461551DMG1
CSB-EP461551DMG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein hedgehog (hh) CSB-YP461552DMG
CSB-EP461552DMG
CSB-BP461552DMG
CSB-MP461552DMG
CSB-EP461552DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein cereblon homolog (GM23853), partial CSB-YP461553DMG
CSB-EP461553DMG
CSB-BP461553DMG
CSB-MP461553DMG
CSB-EP461553DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein teflon (tef), partial CSB-YP461554DMG
CSB-EP461554DMG
CSB-BP461554DMG
CSB-MP461554DMG
CSB-EP461554DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein crossbronx-like (GM19805) CSB-YP461555DMG
CSB-EP461555DMG
CSB-BP461555DMG
CSB-MP461555DMG
CSB-EP461555DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein crossbronx (cbx) CSB-YP461556DMG
CSB-EP461556DMG
CSB-BP461556DMG
CSB-MP461556DMG
CSB-EP461556DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Interference hedgehog (iHog), partial CSB-YP461557DMG
CSB-EP461557DMG
CSB-BP461557DMG
CSB-MP461557DMG
CSB-EP461557DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Regulator of telomere elongation helicase 1 homolog (GM12432), partial CSB-YP461558DMG
CSB-EP461558DMG
CSB-BP461558DMG
CSB-MP461558DMG
CSB-EP461558DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein Turandot F (TotF) CSB-YP461559DMG
CSB-EP461559DMG
CSB-BP461559DMG
CSB-MP461559DMG
CSB-EP461559DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Cytosolic Fe-S cluster assembly factor NUBP2 homolog (GM22307) CSB-YP461560DMG
CSB-EP461560DMG
CSB-BP461560DMG
CSB-MP461560DMG
CSB-EP461560DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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