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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia tasmaniensis UPF0234 protein ETA_25210 (ETA_25210) CSB-YP462862ENB
CSB-EP462862ENB
CSB-BP462862ENB
CSB-MP462862ENB
CSB-EP462862ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP462863ENB
CSB-EP462863ENB
CSB-BP462863ENB
CSB-MP462863ENB
CSB-EP462863ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis HTH-type transcriptional regulator iscR (iscR) CSB-YP462864ENB
CSB-EP462864ENB
CSB-BP462864ENB
CSB-MP462864ENB
CSB-EP462864ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP462865ENB
CSB-EP462865ENB
CSB-BP462865ENB
CSB-MP462865ENB
CSB-EP462865ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Acireductone dioxygenase (mtnD) CSB-YP462866ENB
CSB-EP462866ENB
CSB-BP462866ENB
CSB-MP462866ENB
CSB-EP462866ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP462867ENB1
CSB-EP462867ENB1
CSB-BP462867ENB1
CSB-MP462867ENB1
CSB-EP462867ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Fatty acid metabolism regulator protein (fadR) CSB-YP462868ENB
CSB-EP462868ENB
CSB-BP462868ENB
CSB-MP462868ENB
CSB-EP462868ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Aspartate--tRNA ligase (aspS) CSB-YP462869ENB
CSB-EP462869ENB
CSB-BP462869ENB
CSB-MP462869ENB
CSB-EP462869ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP462870ENB
CSB-EP462870ENB
CSB-BP462870ENB
CSB-MP462870ENB
CSB-EP462870ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Potassium-transporting ATPase C chain (kdpC), partial CSB-YP462871ENB
CSB-EP462871ENB
CSB-BP462871ENB
CSB-MP462871ENB
CSB-EP462871ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 3-dehydroquinate synthase (aroB) CSB-YP462872ENB
CSB-EP462872ENB
CSB-BP462872ENB
CSB-MP462872ENB
CSB-EP462872ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0270 protein ETA_31870 (ETA_31870) CSB-YP462873ENB
CSB-EP462873ENB
CSB-BP462873ENB
CSB-MP462873ENB
CSB-EP462873ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Sulfurtransferase TusD (tusD) CSB-YP462874ENB
CSB-EP462874ENB
CSB-BP462874ENB
CSB-MP462874ENB
CSB-EP462874ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S14 (rpsN) CSB-YP462875ENB
CSB-EP462875ENB
CSB-BP462875ENB
CSB-MP462875ENB
CSB-EP462875ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L16 (rplP) CSB-YP462876ENB
CSB-EP462876ENB
CSB-BP462876ENB
CSB-MP462876ENB
CSB-EP462876ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Shikimate dehydrogenase (aroE) CSB-YP462877ENB
CSB-EP462877ENB
CSB-BP462877ENB
CSB-MP462877ENB
CSB-EP462877ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0263 protein ETA_15890 (ETA_15890) CSB-YP462878ENB
CSB-EP462878ENB
CSB-BP462878ENB
CSB-MP462878ENB
CSB-EP462878ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Probable cytosol aminopeptidase (pepA), partial CSB-YP462879ENB
CSB-EP462879ENB
CSB-BP462879ENB
CSB-MP462879ENB
CSB-EP462879ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis U3 small nucleolar RNA-associated protein 25 (utp25), partial CSB-YP462880FHT
CSB-EP462880FHT
CSB-BP462880FHT
CSB-MP462880FHT
CSB-EP462880FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Mannitol 2-dehydrogenase (PTRG_03680), partial CSB-YP462881FHT
CSB-EP462881FHT
CSB-BP462881FHT
CSB-MP462881FHT
CSB-EP462881FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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