Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum Aspartate 1-decarboxylase (panD) CSB-YP462782DUF
CSB-EP462782DUF
CSB-BP462782DUF
CSB-MP462782DUF
CSB-EP462782DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP462783DUF
CSB-EP462783DUF
CSB-BP462783DUF
CSB-MP462783DUF
CSB-EP462783DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S20 (rpsT) CSB-YP462784DUF
CSB-EP462784DUF
CSB-BP462784DUF
CSB-MP462784DUF
CSB-EP462784DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0210 protein CLH_1879 (CLH_1879) CSB-YP462785DUF
CSB-EP462785DUF
CSB-BP462785DUF
CSB-MP462785DUF
CSB-EP462785DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP462786DUF
CSB-EP462786DUF
CSB-BP462786DUF
CSB-MP462786DUF
CSB-EP462786DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP462787DUF
CSB-EP462787DUF
CSB-BP462787DUF
CSB-MP462787DUF
CSB-EP462787DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Putative Holliday junction resolvase (CLH_1127) CSB-YP462788DUF
CSB-EP462788DUF
CSB-BP462788DUF
CSB-MP462788DUF
CSB-EP462788DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0246 protein CLH_2088 (CLH_2088) CSB-YP462789DUF
CSB-EP462789DUF
CSB-BP462789DUF
CSB-MP462789DUF
CSB-EP462789DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0122 protein CLH_1195 (CLH_1195) CSB-YP462790DUF
CSB-EP462790DUF
CSB-BP462790DUF
CSB-MP462790DUF
CSB-EP462790DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0102 protein CLH_1204 (CLH_1204) CSB-YP462791DUF
CSB-EP462791DUF
CSB-BP462791DUF
CSB-MP462791DUF
CSB-EP462791DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosome maturation factor RimP (rimP) CSB-YP462792DUF
CSB-EP462792DUF
CSB-BP462792DUF
CSB-MP462792DUF
CSB-EP462792DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S15 (rpsO) CSB-YP462793DUF
CSB-EP462793DUF
CSB-BP462793DUF
CSB-MP462793DUF
CSB-EP462793DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, partial CSB-YP462794DUF
CSB-EP462794DUF
CSB-BP462794DUF
CSB-MP462794DUF
CSB-EP462794DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP462795DUF
CSB-EP462795DUF
CSB-BP462795DUF
CSB-MP462795DUF
CSB-EP462795DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor P (efp) CSB-YP462796DUF
CSB-EP462796DUF
CSB-BP462796DUF
CSB-MP462796DUF
CSB-EP462796DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0061 protein CLH_1334 (CLH_1334) CSB-YP462797DUF
CSB-EP462797DUF
CSB-BP462797DUF
CSB-MP462797DUF
CSB-EP462797DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Dihydrodipicolinate reductase (dapB) CSB-YP462798DUF
CSB-EP462798DUF
CSB-BP462798DUF
CSB-MP462798DUF
CSB-EP462798DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamate--tRNA ligase (gltX) CSB-YP462799DUF
CSB-EP462799DUF
CSB-BP462799DUF
CSB-MP462799DUF
CSB-EP462799DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Peptide chain release factor 2 (prfB) CSB-YP462800FPP
CSB-EP462800FPP
CSB-BP462800FPP
CSB-MP462800FPP
CSB-EP462800FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. GTPase Der (der) CSB-YP462801FPP
CSB-EP462801FPP
CSB-BP462801FPP
CSB-MP462801FPP
CSB-EP462801FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>