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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pyrenophora tritici-repentis Nucleolar protein 9 (nop9), partial CSB-YP462882FHT
CSB-EP462882FHT
CSB-BP462882FHT
CSB-MP462882FHT
CSB-EP462882FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Lipoyl synthase, mitochondrial (PTRG_06961) CSB-YP462883FHT
CSB-EP462883FHT
CSB-BP462883FHT
CSB-MP462883FHT
CSB-EP462883FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Probable Xaa-Pro aminopeptidase PTRG_10574 (PTRG_10574), partial CSB-YP462884FHT
CSB-EP462884FHT
CSB-BP462884FHT
CSB-MP462884FHT
CSB-EP462884FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oedogonium cardiacum ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP462885ODX1
CSB-EP462885ODX1
CSB-BP462885ODX1
CSB-MP462885ODX1
CSB-EP462885ODX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oedogonium cardiacum Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP462886ODX
CSB-EP462886ODX
CSB-BP462886ODX
CSB-MP462886ODX
CSB-EP462886ODX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oedogonium cardiacum 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP462887ODX
CSB-EP462887ODX
CSB-BP462887ODX
CSB-MP462887ODX
CSB-EP462887ODX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo Protein translocase subunit SecY (secY), partial CSB-YP462888HWH
CSB-EP462888HWH
CSB-BP462888HWH
CSB-MP462888HWH
CSB-EP462888HWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP462889FEC
CSB-EP462889FEC
CSB-BP462889FEC
CSB-MP462889FEC
CSB-EP462889FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale Photosystem I reaction center subunit IX (psaJ), partial CSB-YP462890FEC
CSB-EP462890FEC
CSB-BP462890FEC
CSB-MP462890FEC
CSB-EP462890FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP462891FEC1
CSB-EP462891FEC1
CSB-BP462891FEC1
CSB-MP462891FEC1
CSB-EP462891FEC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP462892FEC
CSB-EP462892FEC
CSB-BP462892FEC
CSB-MP462892FEC
CSB-EP462892FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Photosystem II reaction center protein Z (psbZ), partial CSB-YP462893WBL1
CSB-EP462893WBL1
CSB-BP462893WBL1
CSB-MP462893WBL1
CSB-EP462893WBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP462894WBL1
CSB-EP462894WBL1
CSB-BP462894WBL1
CSB-MP462894WBL1
CSB-EP462894WBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Photosystem I reaction center subunit VIII (psaI), partial CSB-YP462895WBL
CSB-EP462895WBL
CSB-BP462895WBL
CSB-MP462895WBL
CSB-EP462895WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Cytochrome b6 (petB), partial CSB-YP462896WBL1
CSB-EP462896WBL1
CSB-BP462896WBL1
CSB-MP462896WBL1
CSB-EP462896WBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Drosulfakinins (Dsk) CSB-YP462897DMG
CSB-EP462897DMG
CSB-BP462897DMG
CSB-MP462897DMG
CSB-EP462897DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Whitewater arroyo virus RNA-directed RNA polymerase L (L), partial CSB-YP462898WBM
CSB-EP462898WBM
CSB-BP462898WBM
CSB-MP462898WBM
CSB-EP462898WBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative major head protein (orf47) CSB-YP462899FLO
CSB-EP462899FLO
CSB-BP462899FLO
CSB-MP462899FLO
CSB-EP462899FLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphatidylinositol:ceramide inositolphosphotransferase (SLS1), partial CSB-YP462900TQV1
CSB-EP462900TQV1
CSB-BP462900TQV1
CSB-MP462900TQV1
CSB-EP462900TQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops marajoensis Phospholipase A2, basic 3 CSB-YP462901BUY
CSB-EP462901BUY
CSB-BP462901BUY
CSB-MP462901BUY
CSB-EP462901BUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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