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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma arthritidis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP463182MWD
CSB-EP463182MWD
CSB-BP463182MWD
CSB-MP463182MWD
CSB-EP463182MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis 50S ribosomal protein L36 (rpmJ) CSB-YP463183MWD
CSB-EP463183MWD
CSB-BP463183MWD
CSB-MP463183MWD
CSB-EP463183MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis 50S ribosomal protein L29 (rpmC) CSB-YP463184MWD
CSB-EP463184MWD
CSB-BP463184MWD
CSB-MP463184MWD
CSB-EP463184MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP463185MWD
CSB-EP463185MWD
CSB-BP463185MWD
CSB-MP463185MWD
CSB-EP463185MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis Arginine deiminase (arcA) CSB-YP463186MWD
CSB-EP463186MWD
CSB-BP463186MWD
CSB-MP463186MWD
CSB-EP463186MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli DNA gyrase inhibitor YacG (yacG) CSB-YP463187RKL
CSB-EP463187RKL
CSB-BP463187RKL
CSB-MP463187RKL
CSB-EP463187RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Protein CrcB homolog (crcB), partial CSB-YP463188RKL1
CSB-EP463188RKL1
CSB-BP463188RKL1
CSB-MP463188RKL1
CSB-EP463188RKL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Flagellar P-ring protein (flgI) CSB-YP463189RKL
CSB-EP463189RKL
CSB-BP463189RKL
CSB-MP463189RKL
CSB-EP463189RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0283 membrane protein RHECIAT_CH0002430 (RHECIAT_CH0002430), partial CSB-YP463190RKL1
CSB-EP463190RKL1
CSB-BP463190RKL1
CSB-MP463190RKL1
CSB-EP463190RKL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0235 protein RHECIAT_CH0004196 (RHECIAT_CH0004196) CSB-YP463191RKL
CSB-EP463191RKL
CSB-BP463191RKL
CSB-MP463191RKL
CSB-EP463191RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Cobyric acid synthase (cobQ) CSB-YP463192RKL
CSB-EP463192RKL
CSB-BP463192RKL
CSB-MP463192RKL
CSB-EP463192RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli ATP phosphoribosyltransferase (hisG) CSB-YP463193RKL
CSB-EP463193RKL
CSB-BP463193RKL
CSB-MP463193RKL
CSB-EP463193RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP463194RKL
CSB-EP463194RKL
CSB-BP463194RKL
CSB-MP463194RKL
CSB-EP463194RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Chorismate synthase (aroC) CSB-YP463195RKL
CSB-EP463195RKL
CSB-BP463195RKL
CSB-MP463195RKL
CSB-EP463195RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Homoserine kinase (thrB) CSB-YP463196RKL
CSB-EP463196RKL
CSB-BP463196RKL
CSB-MP463196RKL
CSB-EP463196RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Trans-aconitate 2-methyltransferase (tam) CSB-YP463197RKL
CSB-EP463197RKL
CSB-BP463197RKL
CSB-MP463197RKL
CSB-EP463197RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0260 protein RHECIAT_CH0001358 (RHECIAT_CH0001358) CSB-YP463198RKL
CSB-EP463198RKL
CSB-BP463198RKL
CSB-MP463198RKL
CSB-EP463198RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli SsrA-binding protein (smpB) CSB-YP463199RKL
CSB-EP463199RKL
CSB-BP463199RKL
CSB-MP463199RKL
CSB-EP463199RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli C4-dicarboxylate transport protein (dctA), partial CSB-YP463200RKL
CSB-EP463200RKL
CSB-BP463200RKL
CSB-MP463200RKL
CSB-EP463200RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP463201RKL
CSB-EP463201RKL
CSB-BP463201RKL
CSB-MP463201RKL
CSB-EP463201RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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