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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium etli Tryptophan synthase alpha chain (trpA) CSB-YP463202RKL
CSB-EP463202RKL
CSB-BP463202RKL
CSB-MP463202RKL
CSB-EP463202RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 50S ribosomal protein L13 (rplM) CSB-YP463203RKL
CSB-EP463203RKL
CSB-BP463203RKL
CSB-MP463203RKL
CSB-EP463203RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 50S ribosomal protein L33 (rpmG) CSB-YP463204RKL
CSB-EP463204RKL
CSB-BP463204RKL
CSB-MP463204RKL
CSB-EP463204RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Aspartate carbamoyltransferase (pyrB) CSB-YP463205RKL
CSB-EP463205RKL
CSB-BP463205RKL
CSB-MP463205RKL
CSB-EP463205RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP463206RKL
CSB-EP463206RKL
CSB-BP463206RKL
CSB-MP463206RKL
CSB-EP463206RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP463207RKL
CSB-EP463207RKL
CSB-BP463207RKL
CSB-MP463207RKL
CSB-EP463207RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP463208RKL
CSB-EP463208RKL
CSB-BP463208RKL
CSB-MP463208RKL
CSB-EP463208RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 30S ribosomal protein S10 (rpsJ) CSB-YP463209RKL
CSB-EP463209RKL
CSB-BP463209RKL
CSB-MP463209RKL
CSB-EP463209RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 50S ribosomal protein L24 (rplX) CSB-YP463210RKL
CSB-EP463210RKL
CSB-BP463210RKL
CSB-MP463210RKL
CSB-EP463210RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 50S ribosomal protein L30 (rpmD) CSB-YP463211RKL
CSB-EP463211RKL
CSB-BP463211RKL
CSB-MP463211RKL
CSB-EP463211RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Urease subunit beta (ureB) CSB-YP463212RKL
CSB-EP463212RKL
CSB-BP463212RKL
CSB-MP463212RKL
CSB-EP463212RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) CSB-YP463213RKL
CSB-EP463213RKL
CSB-BP463213RKL
CSB-MP463213RKL
CSB-EP463213RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Arginine--tRNA ligase (argS), partial CSB-YP463214RKL
CSB-EP463214RKL
CSB-BP463214RKL
CSB-MP463214RKL
CSB-EP463214RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0173 metal-dependent hydrolase RHECIAT_CH0001941 (RHECIAT_CH0001941) CSB-YP463215RKL
CSB-EP463215RKL
CSB-BP463215RKL
CSB-MP463215RKL
CSB-EP463215RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Adenylosuccinate synthetase (purA) CSB-YP463216RKL
CSB-EP463216RKL
CSB-BP463216RKL
CSB-MP463216RKL
CSB-EP463216RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP463217RKL
CSB-EP463217RKL
CSB-BP463217RKL
CSB-MP463217RKL
CSB-EP463217RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Probable D-serine dehydratase (dsdA) CSB-YP463218RKL
CSB-EP463218RKL
CSB-BP463218RKL
CSB-MP463218RKL
CSB-EP463218RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phosphoglucosamine mutase (glmM) CSB-YP463219RKL
CSB-EP463219RKL
CSB-BP463219RKL
CSB-MP463219RKL
CSB-EP463219RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP463220RKL
CSB-EP463220RKL
CSB-BP463220RKL
CSB-MP463220RKL
CSB-EP463220RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phosphopantetheine adenylyltransferase (coaD) CSB-YP463221RKL
CSB-EP463221RKL
CSB-BP463221RKL
CSB-MP463221RKL
CSB-EP463221RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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