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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila ananassae Sensory neuron membrane protein 1 (Snmp1), partial CSB-YP463142DKY
CSB-EP463142DKY
CSB-BP463142DKY
CSB-MP463142DKY
CSB-EP463142DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Succinate dehydrogenase assembly factor 2-A, mitochondrial (GG10665), partial CSB-YP463143DLK
CSB-EP463143DLK
CSB-BP463143DLK
CSB-MP463143DLK
CSB-EP463143DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit F-2 (eIF3-S5-2) CSB-YP463144DLK
CSB-EP463144DLK
CSB-BP463144DLK
CSB-MP463144DLK
CSB-EP463144DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein teflon (tef), partial CSB-YP463145DLK
CSB-EP463145DLK
CSB-BP463145DLK
CSB-MP463145DLK
CSB-EP463145DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta WD repeat-containing protein 48 homolog (GG22678), partial CSB-YP463146DLK
CSB-EP463146DLK
CSB-BP463146DLK
CSB-MP463146DLK
CSB-EP463146DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta NAD (P)H-hydrate epimerase CSB-YP463147DLK
CSB-EP463147DLK
CSB-BP463147DLK
CSB-MP463147DLK
CSB-EP463147DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (GG17526) CSB-YP463148DLK
CSB-EP463148DLK
CSB-BP463148DLK
CSB-MP463148DLK
CSB-EP463148DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Forkhead box protein O (foxo), partial CSB-YP463149DLK
CSB-EP463149DLK
CSB-BP463149DLK
CSB-MP463149DLK
CSB-EP463149DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Structure-specific endonuclease subunit SLX1 homolog (slx1) CSB-YP463150DLK
CSB-EP463150DLK
CSB-BP463150DLK
CSB-MP463150DLK
CSB-EP463150DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein Turandot B1 (TotB1) CSB-YP463151DLK
CSB-EP463151DLK
CSB-BP463151DLK
CSB-MP463151DLK
CSB-EP463151DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta WD repeat-containing protein 55 homolog (GG18443) CSB-YP463152DLK
CSB-EP463152DLK
CSB-BP463152DLK
CSB-MP463152DLK
CSB-EP463152DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein phosphatase PTC7 homolog fig (fig) CSB-YP463153DLK
CSB-EP463153DLK
CSB-BP463153DLK
CSB-MP463153DLK
CSB-EP463153DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus 3-dehydroquinate dehydratase (aroQ) CSB-YP463154DQY
CSB-EP463154DQY
CSB-BP463154DQY
CSB-MP463154DQY
CSB-EP463154DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Glucosamine-6-phosphate deaminase (nagB) CSB-YP463155DQY
CSB-EP463155DQY
CSB-BP463155DQY
CSB-MP463155DQY
CSB-EP463155DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Protein translocase subunit SecA (secA), partial CSB-YP463156DQY
CSB-EP463156DQY
CSB-BP463156DQY
CSB-MP463156DQY
CSB-EP463156DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Aspartate 1-decarboxylase (panD) CSB-YP463157DQY
CSB-EP463157DQY
CSB-BP463157DQY
CSB-MP463157DQY
CSB-EP463157DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP463158DQY
CSB-EP463158DQY
CSB-BP463158DQY
CSB-MP463158DQY
CSB-EP463158DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Deoxycytidine triphosphate deaminase (dcd) CSB-YP463159DQY
CSB-EP463159DQY
CSB-BP463159DQY
CSB-MP463159DQY
CSB-EP463159DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Homoserine O-acetyltransferase (metX) CSB-YP463160DQY
CSB-EP463160DQY
CSB-BP463160DQY
CSB-MP463160DQY
CSB-EP463160DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Methylglyoxal synthase (mgsA) CSB-YP463161DQY
CSB-EP463161DQY
CSB-BP463161DQY
CSB-MP463161DQY
CSB-EP463161DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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