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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phenylobacterium zucineum Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP463582EUP
CSB-EP463582EUP
CSB-BP463582EUP
CSB-MP463582EUP
CSB-EP463582EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP463583EUP
CSB-EP463583EUP
CSB-BP463583EUP
CSB-MP463583EUP
CSB-EP463583EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP463584NGB
CSB-EP463584NGB
CSB-BP463584NGB
CSB-MP463584NGB
CSB-EP463584NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 30S ribosomal protein S15 (rpsO) CSB-YP463585NGB
CSB-EP463585NGB
CSB-BP463585NGB
CSB-MP463585NGB
CSB-EP463585NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP463586NGB
CSB-EP463586NGB
CSB-BP463586NGB
CSB-MP463586NGB
CSB-EP463586NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Uracil phosphoribosyltransferase (upp) CSB-YP463587NGB
CSB-EP463587NGB
CSB-BP463587NGB
CSB-MP463587NGB
CSB-EP463587NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae UPF0246 protein NGK_0633 (NGK_0633) CSB-YP463588NGB
CSB-EP463588NGB
CSB-BP463588NGB
CSB-MP463588NGB
CSB-EP463588NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Ribose-5-phosphate isomerase A (rpiA) CSB-YP463589NGB
CSB-EP463589NGB
CSB-BP463589NGB
CSB-MP463589NGB
CSB-EP463589NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Elongation factor P (efp) CSB-YP463590NGB
CSB-EP463590NGB
CSB-BP463590NGB
CSB-MP463590NGB
CSB-EP463590NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Protein CrcB homolog (crcB), partial CSB-YP463591NGB1
CSB-EP463591NGB1
CSB-BP463591NGB1
CSB-MP463591NGB1
CSB-EP463591NGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae UPF0250 protein NGK_1021 (NGK_1021) CSB-YP463592NGB
CSB-EP463592NGB
CSB-BP463592NGB
CSB-MP463592NGB
CSB-EP463592NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Enolase (eno) CSB-YP463593NGB
CSB-EP463593NGB
CSB-BP463593NGB
CSB-MP463593NGB
CSB-EP463593NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP463594NGB
CSB-EP463594NGB
CSB-BP463594NGB
CSB-MP463594NGB
CSB-EP463594NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae tRNA (guanine-N (7)-)-methyltransferase CSB-YP463595NGB
CSB-EP463595NGB
CSB-BP463595NGB
CSB-MP463595NGB
CSB-EP463595NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Chorismate synthase (aroC) CSB-YP463596NGB
CSB-EP463596NGB
CSB-BP463596NGB
CSB-MP463596NGB
CSB-EP463596NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae D-tyrosyl-tRNA (Tyr) deacylase CSB-YP463597NGB
CSB-EP463597NGB
CSB-BP463597NGB
CSB-MP463597NGB
CSB-EP463597NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L27 (rpmA) CSB-YP463598NGB
CSB-EP463598NGB
CSB-BP463598NGB
CSB-MP463598NGB
CSB-EP463598NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP463599NGB
CSB-EP463599NGB
CSB-BP463599NGB
CSB-MP463599NGB
CSB-EP463599NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP463600NGB
CSB-EP463600NGB
CSB-BP463600NGB
CSB-MP463600NGB
CSB-EP463600NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Protein smg homolog (smg) CSB-YP463601NGB
CSB-EP463601NGB
CSB-BP463601NGB
CSB-MP463601NGB
CSB-EP463601NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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