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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila simulans Eukaryotic translation initiation factor 3 subunit G-1 (eIF3-S4-1) CSB-YP463542DMJ
CSB-EP463542DMJ
CSB-BP463542DMJ
CSB-MP463542DMJ
CSB-EP463542DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Interference hedgehog (iHog), partial CSB-YP463543DMR
CSB-EP463543DMR
CSB-BP463543DMR
CSB-MP463543DMR
CSB-EP463543DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Ribosome biogenesis protein BOP1 homolog (GE11901), partial CSB-YP463544DMR
CSB-EP463544DMR
CSB-BP463544DMR
CSB-MP463544DMR
CSB-EP463544DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Facilitated trehalose transporter Tret1 (Tret1), partial CSB-YP463545DMR
CSB-EP463545DMR
CSB-BP463545DMR
CSB-MP463545DMR
CSB-EP463545DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Adenylate kinase 2, mitochondrial (Adk2) CSB-YP463546DMR
CSB-EP463546DMR
CSB-BP463546DMR
CSB-MP463546DMR
CSB-EP463546DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Cytosolic Fe-S cluster assembly factor NUBP2 homolog 2 (GE19695) CSB-YP463547DMR
CSB-EP463547DMR
CSB-BP463547DMR
CSB-MP463547DMR
CSB-EP463547DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Protein maelstrom (mael) CSB-YP463548DMR
CSB-EP463548DMR
CSB-BP463548DMR
CSB-MP463548DMR
CSB-EP463548DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit D-1 (eIF-3p66), partial CSB-YP463549DMR
CSB-EP463549DMR
CSB-BP463549DMR
CSB-MP463549DMR
CSB-EP463549DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Serine protease HTRA2, mitochondrial (HtrA2), partial CSB-YP463550DMR1
CSB-EP463550DMR1
CSB-BP463550DMR1
CSB-MP463550DMR1
CSB-EP463550DMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit F-1 (eIF3-S5-1) CSB-YP463551DMR
CSB-EP463551DMR
CSB-BP463551DMR
CSB-MP463551DMR
CSB-EP463551DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba NAD (P)H-hydrate epimerase CSB-YP463552DMR
CSB-EP463552DMR
CSB-BP463552DMR
CSB-MP463552DMR
CSB-EP463552DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba DM7 family protein GE17493 (GE17493), partial CSB-YP463553DMR
CSB-EP463553DMR
CSB-BP463553DMR
CSB-MP463553DMR
CSB-EP463553DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Glutamyl-tRNA (Gln) amidotransferase subunit C, mitochondrial, partial CSB-YP463554DMJ
CSB-EP463554DMJ
CSB-BP463554DMJ
CSB-MP463554DMJ
CSB-EP463554DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein Turandot E (Victoria) CSB-YP463555DMJ
CSB-EP463555DMJ
CSB-BP463555DMJ
CSB-MP463555DMJ
CSB-EP463555DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Breast cancer type 2 susceptibility protein homolog (Brca2), partial CSB-YP463556DMJ
CSB-EP463556DMJ
CSB-BP463556DMJ
CSB-MP463556DMJ
CSB-EP463556DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein crossbronx-like (GD25298) CSB-YP463557DMJ
CSB-EP463557DMJ
CSB-BP463557DMJ
CSB-MP463557DMJ
CSB-EP463557DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Lissencephaly-1 homolog (Lis-1) CSB-YP463558DMJ
CSB-EP463558DMJ
CSB-BP463558DMJ
CSB-MP463558DMJ
CSB-EP463558DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein maelstrom (mael) CSB-YP463559DMJ
CSB-EP463559DMJ
CSB-BP463559DMJ
CSB-MP463559DMJ
CSB-EP463559DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans SOSS complex subunit C homolog (GD14793) CSB-YP463560DMJ
CSB-EP463560DMJ
CSB-BP463560DMJ
CSB-MP463560DMJ
CSB-EP463560DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Microtubule-associated protein Jupiter (Jupiter) CSB-YP463561DMJ
CSB-EP463561DMJ
CSB-BP463561DMJ
CSB-MP463561DMJ
CSB-EP463561DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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