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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria gonorrhoeae Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP463602NGB
CSB-EP463602NGB
CSB-BP463602NGB
CSB-MP463602NGB
CSB-EP463602NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae D-alanine--D-alanine ligase (ddl) CSB-YP463603NGB
CSB-EP463603NGB
CSB-BP463603NGB
CSB-MP463603NGB
CSB-EP463603NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Undecaprenyl-diphosphatase (uppP), partial CSB-YP463604NGB1
CSB-EP463604NGB1
CSB-BP463604NGB1
CSB-MP463604NGB1
CSB-EP463604NGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae UPF0102 protein NGK_2254 (NGK_2254) CSB-YP463605NGB
CSB-EP463605NGB
CSB-BP463605NGB
CSB-MP463605NGB
CSB-EP463605NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L11 (rplK) CSB-YP463606NGB
CSB-EP463606NGB
CSB-BP463606NGB
CSB-MP463606NGB
CSB-EP463606NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L2 (rplB) CSB-YP463607NGB
CSB-EP463607NGB
CSB-BP463607NGB
CSB-MP463607NGB
CSB-EP463607NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae D-amino acid dehydrogenase small subunit (dadA) CSB-YP463608NGB
CSB-EP463608NGB
CSB-BP463608NGB
CSB-MP463608NGB
CSB-EP463608NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP463609NGB
CSB-EP463609NGB
CSB-BP463609NGB
CSB-MP463609NGB
CSB-EP463609NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP463610AZX
CSB-EP463610AZX
CSB-BP463610AZX
CSB-MP463610AZX
CSB-EP463610AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L14 (rplN) CSB-YP463611AZX
CSB-EP463611AZX
CSB-BP463611AZX
CSB-MP463611AZX
CSB-EP463611AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L30 (rpmD) CSB-YP463612AZX
CSB-EP463612AZX
CSB-BP463612AZX
CSB-MP463612AZX
CSB-EP463612AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Peptidyl-tRNA hydrolase (pth) CSB-YP463613AZX
CSB-EP463613AZX
CSB-BP463613AZX
CSB-MP463613AZX
CSB-EP463613AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP463614AZX
CSB-EP463614AZX
CSB-BP463614AZX
CSB-MP463614AZX
CSB-EP463614AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Homoserine O-succinyltransferase (metA) CSB-YP463615AZX
CSB-EP463615AZX
CSB-BP463615AZX
CSB-MP463615AZX
CSB-EP463615AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Lipoprotein signal peptidase (lspA1), partial CSB-YP463616AZX1
CSB-EP463616AZX1
CSB-BP463616AZX1
CSB-MP463616AZX1
CSB-EP463616AZX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Enolase (eno) CSB-YP463617AZX
CSB-EP463617AZX
CSB-BP463617AZX
CSB-MP463617AZX
CSB-EP463617AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP463618AZX
CSB-EP463618AZX
CSB-BP463618AZX
CSB-MP463618AZX
CSB-EP463618AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii UPF0246 protein MADE_1015435 (MADE_1015435) CSB-YP463619AZX
CSB-EP463619AZX
CSB-BP463619AZX
CSB-MP463619AZX
CSB-EP463619AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii DNA repair protein recO (recO) CSB-YP463620AZX
CSB-EP463620AZX
CSB-BP463620AZX
CSB-MP463620AZX
CSB-EP463620AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii UPF0761 membrane protein MADE_1017605/MADE_1018330 (MADE_1017605), partial CSB-YP463621AZX1
CSB-EP463621AZX1
CSB-BP463621AZX1
CSB-MP463621AZX1
CSB-EP463621AZX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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